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Journal of Virology, May 2003, p. 5694-5702, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.5694-5702.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

In Vitro and In Ovo Expression of Chicken Gamma Interferon by a Defective RNA of Avian Coronavirus Infectious Bronchitis Virus

Karen Hackney, Dave Cavanagh, Pete Kaiser, and Paul Britton*

Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, United Kingdom

Received 25 November 2002/ Accepted 28 February 2003

Coronavirus defective RNAs (D-RNAs) have been used for site-directed mutagenesis of coronavirus genomes and for expression of heterologous genes. D-RNA CD-61 derived from the avian coronavirus infectious bronchitis virus (IBV) was used as an RNA vector for the expression of chicken gamma interferon (chIFN-{gamma}). D-RNAs expressing chIFN-{gamma} were shown to be capable of rescue, replication, and packaging into virions in a helper virus-dependent system following electroporation of in vitro-derived T7 RNA transcripts into IBV-infected cells. Secreted chIFN-{gamma}, under the control of an IBV transcription-associated sequence derived from gene 5 of the Beaudette strain, was expressed from two different positions within CD-61 and shown to be biologically active. In addition, following infection of 10-day-old chicken embryos with IBV containing D-RNAs expressing chIFN-{gamma}, the allantoic fluid was shown to contain biologically active chIFN-{gamma}, demonstrating that IBV D-RNAs can express heterologous genes in vivo.

* Corresponding author. Mailing address: Division of Molecular Biology, Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44 1635 578411. Fax: 44 1635 577263. E-mail: paul.britton{at}bbsrc.ac.uk.

Journal of Virology, May 2003, p. 5694-5702, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.5694-5702.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hodgson, T., Britton, P., Cavanagh, D. (2006). Neither the RNA nor the Proteins of Open Reading Frames 3a and 3b of the Coronavirus Infectious Bronchitis Virus Are Essential for Replication. J. Virol. 80: 296-305 [Abstract] [Full Text]  
  • Casais, R., Davies, M., Cavanagh, D., Britton, P. (2005). Gene 5 of the Avian Coronavirus Infectious Bronchitis Virus Is Not Essential for Replication. J. Virol. 79: 8065-8078 [Abstract] [Full Text]  
  • Smith, C. K., Kaiser, P., Rothwell, L., Humphrey, T., Barrow, P. A., Jones, M. A. (2005). Campylobacter jejuni-Induced Cytokine Responses in Avian Cells. Infect. Immun. 73: 2094-2100 [Abstract] [Full Text]  
  • Dove, B., Cavanagh, D., Britton, P. (2004). Presence of an Encephalomyocarditis Virus Internal Ribosome Entry Site Sequence in Avian Infectious Bronchitis Virus Defective RNAs Abolishes Rescue by Helper Virus. J. Virol. 78: 2711-2721 [Abstract] [Full Text]  


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