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Journal of Virology, January 2003, p. 769-775, Vol. 77, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.1.769-775.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rice Grassy Stunt Tenuivirus Nonstructural Protein p5 Interacts with Itself To Form Oligomeric Complexes In Vitro and In Vivo

Pritsana Chomchan,1,{dagger} Shi-Fang Li,2,{ddagger} and Yukio Shirako2*

Graduate School of Agricultural Life Sciences,1 Asian Center for Bioresources and Environmental Sciences (ANESC), University of Tokyo, Tokyo, Japan2

Received 23 August 2002/ Accepted 3 October 2002

We investigated the interaction of Rice grassy stunt tenuivirus (RGSV) nonstructural protein p5, a protein of 22 kDa encoded on vRNA 5, with all 12 RGSV proteins by using a GAL4 transcription activator-based yeast two-hybrid system. The p5 protein interacted only with itself and not with any other viral protein; the interacting domains were localized within the N-terminal 96 amino acids of p5. The p5-p5 interaction was reproduced in an Sos recruitment-mediated yeast two-hybrid system as well in by far-Western blots. Native p5 protein extracted from RGSV-infected rice tissue was detected in a large complex with a molecular mass of approximately 260 kDa composed of 12 molecules of p5 or a p5 oligomer with an unidentified host factor(s).

* Corresponding author. Mailing address: Asian Center for Bioresources and Environmental Sciences (ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone and fax: 81-3-5841-7524. E-mail: shirako{at}ims.u-tokyo.ac.jp.

{dagger} Present address: Division of Biology, California Institute of Technology, Pasadena, CA 91125.

{ddagger} Present address: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100094, People's Republic of China.

Journal of Virology, January 2003, p. 769-775, Vol. 77, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.1.769-775.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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