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Journal of Virology, January 2003, p. 696-700, Vol. 77, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.1.696-700.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Contribution of the Zinc Finger to Zinc and DNA Binding by a Suppressor of Posttranscriptional Gene Silencing

Rene van Wezel,1 Huanting Liu,2 Zirong Wu,3 John Stanley,4 and Yiguo Hong1*

Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ,1 Centre for Biomolecular Science, University of St. Andrews, Fife KY16 9ST,2 Department of Disease and Stress Biology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom,4 Department of Biology, East China Normal University, Shanghai 200062, China3

Received 18 July 2002/ Accepted 1 October 2002

The zinc finger C36-X1-C38-X7-C46-X6-H53 of the nuclearly localized C2 protein of Tomato yellow leaf curl virus China is involved in pathogenicity and suppression of posttranscriptional gene silencing (PTGS). Here, we demonstrate that the zinc finger is indispensable for the C2 protein to bind zinc and DNA. Mutation of cysteine residue C36, C38, or C46 reduced the zinc and DNA binding capacity of C2 protein. When expressed from potato virus X, all three mutants, C2-C36R, C2-C38N, and C2-C46I, tagged with a green fluorescent protein (GFP) were still capable of transporting GFP into but aggregated abnormally in nuclei. Our data establish that zinc- and DNA-binding activity correlates with C2-mediated pathogenesis and PTGS suppression.

* Corresponding author. Mailing address: Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, United Kingdom. Phone: 44 173 284 3833. Fax: 44 173 284 9067. E-mail: yiguo.hong{at}hri.ac.uk.

Journal of Virology, January 2003, p. 696-700, Vol. 77, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.1.696-700.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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