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Journal of Virology, May 2002, p. 4662-4665, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4662-4665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dengue Type 3 Virus in Plasma Is a Population of Closely Related Genomes: Quasispecies

Wei-Kung Wang,1,2* Su-Ru Lin,1 Chao-Min Lee,3 Chwan-Chuen King,4 and Shan-Chwen Chang2

Institute of Microbiology,1 Department of Internal Medicine, College of Medicine,2 Institute of Epidemiology, College of Public Health, National Taiwan University,4 Department of Dentistry, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan3

Received 8 November 2001/ Accepted 23 January 2002

Using reverse transcription-PCR and clonal sequencing of the dengue virus envelope gene derived from the plasma samples of six patients, we reported for the first time that dengue virus circulates as a population of closely related genomes. The extent of sequence diversity varied among patients, with the mean pairwise proportions of difference ranging from 0.21 to 1.67%. Genome-defective viruses were found in 5.8% of the total number of clones analyzed. Our findings on the quasispecies nature of dengue virus and the defective virus in vivo have implications with regard to the pathogenesis of dengue virus.

* Corresponding author. Mailing address: Institute of Microbiology, College of Medicine, National Taiwan University, No. 1, Sec. 1 Jen-Ai Rd., Taipei, Taiwan. Phone: 886-2-2312-3456, ext. 8286. Fax: 886-2-2391-5293. E-mail: wwang60{at}yahoo.com.

Journal of Virology, May 2002, p. 4662-4665, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4662-4665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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