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Journal of Virology, May 2002, p. 4643-4648, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4643-4648.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Murine Sarcoma Virus RNA Packaging Sequence

Department of Medicine, Children's Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts

Received 15 November 2001/ Accepted 29 January 2002

We investigated the features of the Moloney murine sarcoma virus leader sequence necessary for RNA packaging function by using a deletion analysis approach. We found that sequences that extend beyond those characterized genetically in previous reports are important for optimal packaging efficiency. A fragment covering a minimum of four potential stem-loop structures is required for the shortest packaging element compatible with gene transfer. Our results reveal the extent to which each of the segments of the packaging sequence contribute to packaging efficiency.

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