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Journal of Virology, May 2002, p. 4390-4400, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4390-4400.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Human Herpesvirus 8 Glycoprotein B (gB), gH, and gL Can Mediate Cell Fusion

Peter E. Pertel*

Division of Infectious Diseases, Department of Medicine, and Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois

Received 21 September 2001/ Accepted 24 January 2002

Herpesvirus entry into cells and herpesvirus-induced cell fusion are related processes in that virus penetration proceeds by fusion of the viral envelope and cell membrane. To characterize the human herpesvirus 8 (HHV-8) glycoproteins that can mediate cell fusion, a luciferase reporter gene activation assay was used. Chinese hamster ovary (CHO) cells expressing the HHV-8 glycoproteins of interest along with a luciferase reporter gene under the control of the T7 promoter were cocultivated with human cells transfected with T7 RNA polymerase. Because HHV-8 glycoprotein B (gB) expressed in CHO cells localizes to the perinuclear region, a truncated form of gB (designated gBMUT) that lacks putative endocytosis signals was constructed by deletion of the distal 58 amino acids of the cytoplasmic tail. HHV-8 gBMUT was expressed efficiently on the surface of CHO cells. HHV-8 gB, gH, and gL could mediate the fusion of CHO cells with two different human cell types, embryonic kidney cells and B lymphocytes. Substituting gBMUT for gB significantly enhanced the fusion of CHO cells with human embryonic kidney cells but not B lymphocytes. Thus, two human cell types known to be susceptible to HHV-8 entry were also suitable targets for cell fusion induced by HHV-8 gB, gH, and gL. For human embryonic kidney cells and B cells at least, optimal fusion was noted with the expression of all three HHV-8 glycoproteins.

* Mailing address: 676 North St. Clair St., Suite 200, Chicago, IL 60611. Phone: (312) 695-5090. Fax: (312) 695-5088. E-mail: p-pertel{at}northwestern.edu.

Journal of Virology, May 2002, p. 4390-4400, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4390-4400.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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