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Journal of Virology, April 2002, p. 3936-3942, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.3936-3942.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

African Swine Fever Virus IAP-Like Protein Induces the Activation of Nuclear Factor Kappa B

Clara I. Rodríguez, María L. Nogal, Angel L. Carrascosa, María L. Salas, Manuel Fresno, and Yolanda Revilla*

Centro de Biología Molecular "Severo Ochoa," Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain

Received 23 October 2001/ Accepted 10 January 2002

African swine fever virus (ASFV) encodes a homologue of the inhibitor of apoptosis (IAP) that promotes cell survival by controlling the activity of caspase-3. Here we show that ASFV IAP is also able to activate the transcription factor NF-{kappa}B. Thus, transient transfection of the viral IAP increases the activity of an NF-{kappa}B reporter gene in a dose-responsive manner in Jurkat cells. Similarly, stably transfected cells expressing ASFV IAP have elevated basal levels of c-rel, an NF-{kappa}B-dependent gene. NF-{kappa}B complexes in the nucleus were increased in A224L-expressing cells compared with control cells upon stimulation with phorbol myristate acetate (PMA) plus ionomycin. This resulted in greater NF-{kappa}B-dependent promoter activity in ASFV IAP-expressing than in control cells, both in basal conditions and after PMA plus ionophore stimulation. The elevated NF-{kappa}B activity seems to be the consequence of higher I{kappa}B kinase (IKK) basal activity in these cells. The NF-{kappa}B-inducing activity of ASFV IAP was abrogated by an IKK-2 dominant negative mutant and enhanced by expression of tumor necrosis factor receptor-associated factor 2.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa," Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain. Phone: 34-913978486. Fax: 34-913974799. E-mail: yrevilla{at}cbm.uam.es.

Journal of Virology, April 2002, p. 3936-3942, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.3936-3942.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Granja, A. G., Nogal, M. L., Hurtado, C., Vila, V., Carrascosa, A. L., Salas, M. L., Fresno, M., Revilla, Y. (2004). The Viral Protein A238L Inhibits Cyclooxygenase-2 Expression through a Nuclear Factor of Activated T Cell-dependent Transactivation Pathway. J. Biol. Chem. 279: 53736-53746 [Abstract] [Full Text]  
  • Granja, A. G., Nogal, M. L., Hurtado, C., Salas, J., Salas, M. L., Carrascosa, A. L., Revilla, Y. (2004). Modulation of p53 Cellular Function and Cell Death by African Swine Fever Virus. J. Virol. 78: 7165-7174 [Abstract] [Full Text]  


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