This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Young, P. J.
Right arrow Articles by Lorson, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Young, P. J.
Right arrow Articles by Lorson, C. L.

 Previous Article  |  Next Article 

Journal of Virology, April 2002, p. 3892-3904, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.3892-3904.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Minute Virus of Mice NS1 Interacts with the SMN Protein, and They Colocalize in Novel Nuclear Bodies Induced by Parvovirus Infection

Philip J. Young,1 Klaus T. Jensen,2 Lisa R. Burger,2 David J. Pintel,2 and Christian L. Lorson1*

Department of Biology, Arizona State University, Tempe, Arizona 85287,1 Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, Missouri 652122

Received 24 October 2001/ Accepted 10 January 2002

The human survival motor neuron (SMN) gene is the spinal muscular atrophy-determining gene, and a knockout of the murine Smn gene results in preembryonic lethality. Here we show that SMN can directly interact in vitro and in vivo with the large nonstructural protein NS1 of the autonomous parvovirus minute virus of mice (MVM), a protein essential for viral replication and a potent transcriptional activator. Typically, SMN localizes within nuclear Cajal bodies and diffusely in the cytoplasm. Following transient NS1expression, SMN and NS1 colocalize within Cajal bodies. At early time points following parvovirus infection, NS1 fails to colocalize with SMN within Cajal bodies; however, during the course of MVM infection, dramatic nuclear alterations occur. Formerly distinct nuclear bodies such as Cajal bodies, promyelocytic leukemia gene product (PML) oncogenic domains (PODs), speckles, and autonomous parvovirus-associated replication (APAR) bodies are seen aggregating at later points in infection. These newly formed large nuclear bodies (termed SMN-associated APAR bodies) are active sites of viral replication and viral capsid assembly. These results highlight the transient nature of nuclear bodies and their contents and identify a novel nuclear body formed during infection. Furthermore, simple transient expression of the viral nonstructural proteins is insufficient to induce this nuclear reorganization, suggesting that this event is induced specifically by a step in the viral infection process.

* Corresponding author. Mailing address: Department of Biology, Arizona State University, Tempe, AZ 85287. Phone: (480) 965-5444. Fax: (480) 965-2519. E-mail: clorson{at}asu.edu.

Journal of Virology, April 2002, p. 3892-3904, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.3892-3904.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guan, W., Cheng, F., Yoto, Y., Kleiboeker, S., Wong, S., Zhi, N., Pintel, D. J., Qiu, J. (2008). Block to the Production of Full-Length B19 Virus Transcripts by Internal Polyadenylation Is Overcome by Replication of the Viral Genome. J. Virol. 82: 9951-9963 [Abstract] [Full Text]  
  • Engelsma, D., Valle, N., Fish, A., Salome, N., Almendral, J. M., Fornerod, M. (2008). A Supraphysiological Nuclear Export Signal Is Required for Parvovirus Nuclear Export. Mol. Biol. Cell 19: 2544-2552 [Abstract] [Full Text]  
  • Cotmore, S. F., Gottlieb, R. L., Tattersall, P. (2007). Replication Initiator Protein NS1 of the Parvovirus Minute Virus of Mice Binds to Modular Divergent Sites Distributed throughout Duplex Viral DNA. J. Virol. 81: 13015-13027 [Abstract] [Full Text]  
  • Morse, R., Shaw, D. J., Todd, A. G., Young, P. J. (2007). Targeting of SMN to Cajal bodies is mediated by self-association. Hum Mol Genet 16: 2349-2358 [Abstract] [Full Text]  
  • Young, P. J., Newman, A., Jensen, K. T., Burger, L. R., Pintel, D. J., Lorson, C. L. (2005). Minute virus of mice small non-structural protein NS2 localizes within, but is not required for the formation of, Smn-associated autonomous parvovirus-associated replication bodies. J. Gen. Virol. 86: 1009-1014 [Abstract] [Full Text]  
  • Li, J., Werner, E., Hergenhahn, M., Poirey, R., Luo, Z., Rommelaere, J., Jauniaux, J.-C. (2005). Expression Profiling of Human Hepatoma Cells Reveals Global Repression of Genes Involved in Cell Proliferation, Growth, and Apoptosis upon Infection with Parvovirus H-1. J. Virol. 79: 2274-2286 [Abstract] [Full Text]  
  • Armon, C., Brenner, S. R., Horner, R., Feussner, J. R. (2004). Occurrence of amyotrophic lateral sclerosis among Gulf War veterans. Neurology 62: 1027-1029 [Full Text]  
  • Abd-Alla, A., Jousset, F.-X., Li, Y., Fediere, G., Cousserans, F., Bergoin, M. (2004). NS-3 Protein of the Junonia coenia Densovirus Is Essential for Viral DNA Replication in an Ld 652 Cell Line and Spodoptera littoralis Larvae. J. Virol. 78: 790-797 [Abstract] [Full Text]  
  • Daeffler, L., Horlein, R., Rommelaere, J., Nuesch, J. P. F. (2003). Modulation of Minute Virus of Mice Cytotoxic Activities through Site-Directed Mutagenesis within the NS Coding Region. J. Virol. 77: 12466-12478 [Abstract] [Full Text]  
  • Best, S. M., Shelton, J. F., Pompey, J. M., Wolfinbarger, J. B., Bloom, M. E. (2003). Caspase Cleavage of the Nonstructural Protein NS1 Mediates Replication of Aleutian Mink Disease Parvovirus. J. Virol. 77: 5305-5312 [Abstract] [Full Text]  
  • Eichwald, V., Daeffler, L., Klein, M., Rommelaere, J., Salome, N. (2002). The NS2 Proteins of Parvovirus Minute Virus of Mice Are Required for Efficient Nuclear Egress of Progeny Virions in Mouse Cells. J. Virol. 76: 10307-10319 [Abstract] [Full Text]  
  • Young, P. J., Jensen, K. T., Burger, L. R., Pintel, D. J., Lorson, C. L. (2002). Minute Virus of Mice Small Nonstructural Protein NS2 Interacts and Colocalizes with the Smn Protein. J. Virol. 76: 6364-6369 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»