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Journal of Virology, April 2002, p. 3359-3364, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3359-3364.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Replication of Bovine Papillomavirus Type 1 (BPV-1) DNA in Saccharomyces cerevisiae following Infection with BPV-1 Virions

Kong-Nan Zhao and Ian H. Frazer*

Centre for Immunology and Cancer Research, The University of Queensland, Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia

Received 16 October 2001/ Accepted 20 December 2001

Saccharomyces cerevisiae protoplasts exposed to bovine papillomavirus type 1 (BPV-1) virions demonstrated uptake of virions on electron microscopy. S. cerevisiae cells looked larger after exposure to BPV-1 virions, and cell wall regeneration was delayed. Southern blot hybridization of Hirt DNA from cells exposed to BPV-1 virions demonstrated BPV-1 DNA, which could be detected over 80 days of culture and at least 13 rounds of division. Two-dimensional gel analysis of Hirt DNA showed replicative intermediates, confirming that the BPV-1 genome was replicating within S. cerevisiae. Nicked circle, linear, and supercoiled BPV-1 DNA species were observed in Hirt DNA preparations from S. cerevisiae cells infected for over 50 days, and restriction digestion showed fragments hybridizing to BPV-1 in accord with the predicted restriction map for circular BPV-1 episomes. These data suggest that BPV-1 can infect S. cerevisiae and that BPV-1 episomes can replicate in the infected S. cerevisiae cells.

* Corresponding author. Mailing address: Centre for Immunology and Cancer Research, The University of Queensland, Princess Alexandra Hospital, Brisbane QLD 4102, Australia. Phone: 61 7 3240 5315. Fax: 61 7 3240 5946. E-mail: ifrazer{at}medicine.pa.uq.edu.au.

Journal of Virology, April 2002, p. 3359-3364, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3359-3364.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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