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Journal of Virology, April 2002, p. 3179-3188, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3179-3188.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mechanism of Human Immunodeficiency Virus-Induced Complement Expression in Astrocytes and Neurons

Cornelia Speth,1,2* Thomas Schabetsberger,1,2 Iradji Mohsenipour,3 Gabriele Stöckl,1,2 Reinhard Würzner,1,2 Heribert Stoiber,1,2 Cornelia Lass-Flörl,1,2 and Manfred P. Dierich1,2

Institute of Hygiene and Social Medicine,1 Department of Neurosurgery, University of Innsbruck ,3 Ludwig Boltzmann Institute for AIDS Research, Innsbruck, Austria2

Received 4 October 2001/ Accepted 2 January 2002

The cerebral complement system is hypothesized to contribute to neurodegeneration in the pathogenesis of AIDS-associated neurological disorders. Our former results have shown that the human immunodeficiency virus (HIV) strongly induces the synthesis of complement factor C3 in astrocytes. This upregulation explains in vivo data showing elevated complement levels in the cerebrospinal fluid of patients with AIDS-associated neurological symptoms. Since inhibition of complement synthesis and activation in the brain may represent a putative therapeutic goal to prevent virus-induced damage, we analyzed in detail the mechanisms of HIV-induced modulation of C3 expression. HIV-1 increased the C3 levels in astrocyte culture supernatants from 30 to up to 400 ng/ml; signal transduction studies revealed that adenylate cyclase activation with upregulation of cyclic AMP is the central signaling pathway to mediate that increase. Furthermore, activity of protein kinase C is necessary for HIV induction of C3, since inhibition of protein kinase C by prolonged exposure to the phorbol ester tetradecanoyl phorbol acetate partly abolished the HIV effect. The cytokines tumor necrosis factor alpha and gamma interferon were not involved in mediating the HIV-induced C3 upregulation, since neutralizing antibodies had no effect. Besides whole HIV virions, the purified viral proteins Nef and gp41 are biologically active in upregulating C3, whereas Tat, gp120, and gp160 were not able to modulate C3 synthesis. Further experiments revealed that neurons were also able to respond on incubation with HIV with increased C3 synthesis, although the precise pattern was slightly different from that in astrocytes. This strengthens the hypothesis that HIV-induced complement synthesis represents an important mechanism for the pathogenesis of AIDS in the brain.

* Corresponding author. Mailing address: Institute of Hygiene and Social Medicine, University of Innsbruck, Fritz-Pregl-Str. 3, A-6020 Innsbruck, Austria. Phone: 43 512 5073405. Fax: 43 512 5072870. E-mail: cornelia.speth{at}uibk.ac.at.

Journal of Virology, April 2002, p. 3179-3188, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3179-3188.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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