This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shirakata, M.
Right arrow Articles by Yamanashi, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shirakata, M.
Right arrow Articles by Yamanashi, Y.

 Previous Article  |  Next Article 

Journal of Virology, April 2002, p. 3158-3167, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3158-3167.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Immediate-Early Protein IE19 of Human Cytomegalovirus Activates the Origin Recognition Complex I Promoter in a Cooperative Manner with IE72

Masaki Shirakata,1* Masakazu Terauchi,2 Melike Ablikim,1 Ken-Ichi Imadome,1 Kanji Hirai,1,{dagger} Takeshi Aso,2 and Yuji Yamanashi1

Department of Tumor Virology, Division of Virology and Immunology, Medical Research Institute,1 Department of Comprehensive Reproductive Medicine, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo, Tokyo 113-8510, Japan2

Received 10 October 2001/ Accepted 29 December 2001

The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated an 0.65-kb cDNA from HCMV-infected fibroblast RNA, which does not correspond to any known MIE cDNA. Nucleotide sequencing revealed that the 0.65-kb cDNA is from exons 1, 2, and 3 and part of exon 4, indicating that it is derived from a novel alternatively spliced mRNA of the MIE gene. The cDNA encodes a 172-amino-acid polypeptide, termed IE19, which corresponds to an IE72 variant with an internal deletion from Val86 to Pro404 and appears as a band at 38 kDa on a sodium dodecyl sulfate-polyacrylamide gel. IE19 mRNA was expressed at a low level in the immediate-early, early, and late period of viral infection. IE19 was localized in nuclei, and a transient-expression assay revealed that IE19 enhances IE72-dependent activation of the HsOrc1 promoter, which is identified here as an IE72 target promoter. Another MIE protein, IE86, activated the same promoter but only weakly compared to IE72, and coexpression of IE19 did not alter the IE86-mediated transcriptional activation. In addition, IE19 did not enhance the IE72-dependent activation of the HCMV UL54 promoter. These results suggest that IE19 is a transcriptional coactivator that works with IE72.

* Corresponding author. Mailing address: Department of Tumor Virology, Medical Research Institute, Tokyo Medical and Dental University, Bldg. D, Rm. D226, Yushima 1-5-45, Bunkyo, Tokyo 113-8510, Japan. Phone: (81)-3-5803-5815. Fax: (81)-3-5803-0241. E-mail: shirakata.creg{at}mri.tmd.ac.jp.

{dagger} Deceased.

Journal of Virology, April 2002, p. 3158-3167, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3158-3167.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • White, E. A., Del Rosario, C. J., Sanders, R. L., Spector, D. H. (2007). The IE2 60-Kilodalton and 40-Kilodalton Proteins Are Dispensable for Human Cytomegalovirus Replication but Are Required for Efficient Delayed Early and Late Gene Expression and Production of Infectious Virus. J. Virol. 81: 2573-2583 [Abstract] [Full Text]  
  • White, E. A., Spector, D. H. (2005). Exon 3 of the Human Cytomegalovirus Major Immediate-Early Region Is Required for Efficient Viral Gene Expression and for Cellular Cyclin Modulation. J. Virol. 79: 7438-7452 [Abstract] [Full Text]  
  • Reinhardt, J., Smith, G. B., Himmelheber, C. T., Azizkhan-Clifford, J., Mocarski, E. S. (2005). The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication. J. Virol. 79: 225-233 [Abstract] [Full Text]  
  • Awasthi, S., Isler, J. A., Alwine, J. C. (2004). Analysis of Splice Variants of the Immediate-Early 1 Region of Human Cytomegalovirus. J. Virol. 78: 8191-8200 [Abstract] [Full Text]  
  • Nevels, M., Brune, W., Shenk, T. (2004). SUMOylation of the Human Cytomegalovirus 72-Kilodalton IE1 Protein Facilitates Expression of the 86-Kilodalton IE2 Protein and Promotes Viral Replication. J. Virol. 78: 7803-7812 [Abstract] [Full Text]  
  • Adair, R., Liebisch, G. W., Colberg-Poley, A. M. (2003). Complex alternative processing of human cytomegalovirus UL37 pre-mRNA. J. Gen. Virol. 84: 3353-3358 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»