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Journal of Virology, March 2002, p. 3078-3083, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3078-3083.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Phosphoprotein (P) Binding Site Resides in the N Terminus of the L Polymerase Subunit of Sendai Virus

David E. Holmes and Sue A. Moyer*

Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 32610

Received 8 October 2001/ Accepted 18 December 2001

Sendai virus encodes an RNA-dependent RNA polymerase which is composed of the L and P proteins. Site-directed mutagenesis of the N terminus of L has identified amino acids important for binding P. Seven of nine mutants in amino acids 1 to 350 of Sendai L lost the ability to bind to Sendai P, although they were still able to bind the viral C protein. Loss of P binding correlated with the loss of all RNA synthesis activities. Two L mutants gave limited P-L complex formation and limited viral transcription and replication.

* Corresponding author. Mailing address: University of Florida College of Medicine, Department of Molecular Genetics and Microbiology, 1600 S.W. Archer Rd., P.O. Box 100266, Gainesville, FL 32610-0266. Phone: (352) 392-3131. Fax: (352) 846-2042. E-mail: smoyer{at}ufl.edu.

Journal of Virology, March 2002, p. 3078-3083, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3078-3083.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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