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Journal of Virology, March 2002, p. 3072-3077, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3072-3077.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Human B Cells Immortalized with Epstein-Barr Virus Upregulate CCR6 and CCR10 and Downregulate CXCR4 and CXCR5

Takashi Nakayama,1 Ryuichi Fujisawa,1 Dai Izawa,1 Kunio Hieshima,1 Kenzo Takada,2 and Osamu Yoshie1*

Department of Microbiology, Kinki University School of Medicine, Osaka-Sayama, Osaka 589-8511,1 Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan2

Received 1 October 2001/ Accepted 20 December 2001

Compared to peripheral blood resting B cells, Epstein-Barr virus (EBV)-immortalized B cells consistently express CCR6 and CCR10 at high levels and CXCR4 and CXCR5 at low levels. Accordingly, these cells vigorously responded to the ligands of CCR6 and CCR10 but not to those of CXCR4 and CXCR5. In a human EBV-negative B-cell line, BJAB, stable expression of EBNA2 upregulated CCR6, while stable expression of EBNA2 as well as LMP1 downregulated CXCR4. On the other hand, upregulation of CCR10 or downregulation of CXCR5 was not induced in BJAB by stable expression of EBNA2 or LMP1. Thus, these changes may be due to a plasmablast-like stage of B-cell differentiation fixed by EBV immortalization. EBV-infected B cells in infectious mononucleosis are known to avoid germinal centers and accumulate under the mucosal surfaces. EBV-associated opportunistic lymphomas also tend to occur in extranodal sites. These preferred sites of in vivo localization are consistent with the unique profile of chemokine receptor expression exhibited by EBV-immortalized B cells.

* Corresponding author. Mailing address: Department of Microbiology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan. Phone and fax: 81-(0)72-367-3606. E-mail: o.yoshie{at}med.kindai.ac.jp.

Journal of Virology, March 2002, p. 3072-3077, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3072-3077.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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