This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baric, R. S.
Right arrow Articles by Moe, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baric, R. S.
Right arrow Articles by Moe, C. L.

 Previous Article  |  Next Article 

Journal of Virology, March 2002, p. 3023-3030, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3023-3030.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons

Ralph S. Baric,1,2* Boyd Yount,1 Lisa Lindesmith,1 Patrick R. Harrington,2 Shermalyn R. Greene,1 Fan-Chen Tseng,1 Nancy Davis,2 Robert E. Johnston,2 David G. Klapper,2 and Christine L. Moe1

Department of Epidemiology, Program in Infectious Diseases, School of Public Health,1 Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-74002

Received 8 May 2001/ Accepted 11 December 2001

The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells.

* Corresponding author. Mailing address: Department of Epidemiology, Program in Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7400. Phone: (919) 966-3895. Fax: (919) 966-2089. E-mail: rbaric{at}sph.unc.edu.

Journal of Virology, March 2002, p. 3023-3030, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3023-3030.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cannon, J. L., Lindesmith, L. C., Donaldson, E. F., Saxe, L., Baric, R. S., Vinje, J. (2009). Herd Immunity to GII.4 Noroviruses Is Supported by Outbreak Patient Sera. J. Virol. 83: 5363-5374 [Abstract] [Full Text]  
  • LoBue, A. D., Thompson, J. M., Lindesmith, L., Johnston, R. E., Baric, R. S. (2009). Alphavirus-Adjuvanted Norovirus-Like Particle Vaccines: Heterologous, Humoral, and Mucosal Immune Responses Protect against Murine Norovirus Challenge. J. Virol. 83: 3212-3227 [Abstract] [Full Text]  
  • Sims, A. C., Baric, R. S., Yount, B., Burkett, S. E., Collins, P. L., Pickles, R. J. (2005). Severe Acute Respiratory Syndrome Coronavirus Infection of Human Ciliated Airway Epithelia: Role of Ciliated Cells in Viral Spread in the Conducting Airways of the Lungs. J. Virol. 79: 15511-15524 [Abstract] [Full Text]  
  • Yount, B., Roberts, R. S., Sims, A. C., Deming, D., Frieman, M. B., Sparks, J., Denison, M. R., Davis, N., Baric, R. S. (2005). Severe Acute Respiratory Syndrome Coronavirus Group-Specific Open Reading Frames Encode Nonessential Functions for Replication in Cell Cultures and Mice. J. Virol. 79: 14909-14922 [Abstract] [Full Text]  
  • Lindesmith, L., Moe, C., LePendu, J., Frelinger, J. A., Treanor, J., Baric, R. S. (2005). Cellular and Humoral Immunity following Snow Mountain Virus Challenge. J. Virol. 79: 2900-2909 [Abstract] [Full Text]  
  • Burton-MacLeod, J. A., Kane, E. M., Beard, R. S., Hadley, L. A., Glass, R. I., Ando, T. (2004). Evaluation and Comparison of Two Commercial Enzyme-Linked Immunosorbent Assay Kits for Detection of Antigenically Diverse Human Noroviruses in Stool Samples. J. Clin. Microbiol. 42: 2587-2595 [Abstract] [Full Text]  
  • Harrington, P. R., Vinje, J., Moe, C. L., Baric, R. S. (2004). Norovirus Capture with Histo-Blood Group Antigens Reveals Novel Virus-Ligand Interactions. J. Virol. 78: 3035-3045 [Abstract] [Full Text]  
  • Harrington, P. R., Lindesmith, L., Yount, B., Moe, C. L., Baric, R. S. (2002). Binding of Norwalk Virus-Like Particles to ABH Histo-Blood Group Antigens Is Blocked by Antisera from Infected Human Volunteers or Experimentally Vaccinated Mice. J. Virol. 76: 12335-12343 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»