This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhao, X.-Q. Articles by Rinaldo, C. R. Search for Related Content PubMed PubMed Citation Articles by Zhao, X.-Q. Articles by Rinaldo, C. R., Jr.

 Previous Article  |  Next Article 

Journal of Virology, March 2002, p. 3007-3014, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.3007-3014.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Induction of Anti-Human Immunodeficiency Virus Type 1 (HIV-1) CD8⁺ and CD4⁺ T-Cell Reactivity by Dendritic Cells Loaded with HIV-1 X4-Infected Apoptotic Cells

Department of Infectious Diseases and Microbiology, Graduate School of Public Health,¹ Departments of Pathology,² Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 ,³ Immunex Corporation, Seattle, Washington 98101⁴

Received 11 July 2001/ Accepted 29 November 2001

T-cell responses to X4 strains of human immunodeficiency virus type 1 (HIV-1) are considered important in controlling progression of HIV-1 infection. We investigated the ability of dendritic cells (DC) and various forms of HIV-1 X4 antigen to induce anti-HIV-1 T-cell responses in autologous peripheral blood mononuclear cells from HIV-1-infected persons. Immature DC loaded with HIV-1 IIIB-infected, autologous, apoptotic CD8^- cells and matured with CD40 ligand induced gamma interferon production in autologous CD8⁺ and CD4⁺ T cells. In contrast, mature DC loaded with HIV-1 IIIB-infected, necrotic cells or directly infected with cell-free HIV-1 IIIB were poorly immunogenic. Thus, HIV-1-infected cells undergoing apoptosis serve as a rich source of X4 antigen for CD8⁺ and CD4⁺ T cells by DC. This may be an important mechanism of HIV-1 immunogenicity and provides a strategy for immunotherapy of HIV-1-infected patients on combination antiretroviral therapy.

This article has been cited by other articles:

• Frank, I., Stossel, H., Gettie, A., Turville, S. G., Bess, J. W. Jr., Lifson, J. D., Sivin, I., Romani, N., Robbiani, M. (2008). A Fusion Inhibitor Prevents Spread of Immunodeficiency Viruses, but Not Activation of Virus-Specific T Cells, by Dendritic Cells. J. Virol. 82: 5329-5339 [Abstract] [Full Text]  
• Van Gulck, E. R., Vanham, G., Heyndrickx, L., Coppens, S., Vereecken, K., Atkinson, D., Florence, E., Kint, I., Berneman, Z. N., Tendeloo, V. V. (2008). Efficient In Vitro Expansion of Human Immunodeficiency Virus (HIV)-Specific T-Cell Responses by gag mRNA-Electroporated Dendritic Cells from Treated and Untreated HIV Type 1-Infected Individuals. J. Virol. 82: 3561-3573 [Abstract] [Full Text]  
• Moris, A., Pajot, A., Blanchet, F., Guivel-Benhassine, F., Salcedo, M., Schwartz, O. (2006). Dendritic cells and HIV-specific CD4+ T cells: HIV antigen presentation, T-cell activation, and viral transfer. Blood 108: 1643-1651 [Abstract] [Full Text]  
• Van Gulck, E. R. A., Ponsaerts, P., Heyndrickx, L., Vereecken, K., Moerman, F., De Roo, A., Colebunders, R., Van den Bosch, G., Van Bockstaele, D. R., Van Tendeloo, V. F. I., Allard, S., Verrier, B., Maranon, C., Hoeffel, G., Hosmalin, A., Berneman, Z. N., Vanham, G. (2006). Efficient stimulation of HIV-1-specific T cells using dendritic cells electroporated with mRNA encoding autologous HIV-1 Gag and Env proteins. Blood 107: 1818-1827 [Abstract] [Full Text]  
• Nair, M. P. N., Mahajan, S. D., Schwartz, S. A., Reynolds, J., Whitney, R., Bernstein, Z., Chawda, R. P., Sykes, D., Hewitt, R., Hsiao, C. B. (2005). Cocaine Modulates Dendritic Cell-Specific C Type Intercellular Adhesion Molecule-3-Grabbing Nonintegrin Expression by Dendritic Cells in HIV-1 Patients. J. Immunol. 174: 6617-6626 [Abstract] [Full Text]  
• Maranon, C., Desoutter, J.-F., Hoeffel, G., Cohen, W., Hanau, D., Hosmalin, A. (2004). Dendritic cells cross-present HIV antigens from live as well as apoptotic infected CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 101: 6092-6097 [Abstract] [Full Text]  
• Moris, A., Nobile, C., Buseyne, F., Porrot, F., Abastado, J.-P., Schwartz, O. (2004). DC-SIGN promotes exogenous MHC-I-restricted HIV-1 antigen presentation. Blood 103: 2648-2654 [Abstract] [Full Text]  
• Tsunetsugu-Yokota, Y., Morikawa, Y., Isogai, M., Kawana-Tachikawa, A., Odawara, T., Nakamura, T., Grassi, F., Autran, B., Iwamoto, A. (2003). Yeast-Derived Human Immunodeficiency Virus Type 1 p55gag Virus-Like Particles Activate Dendritic Cells (DCs) and Induce Perforin Expression in Gag-Specific CD8+ T Cells by Cross-Presentation of DCs. J. Virol. 77: 10250-10259 [Abstract] [Full Text]  
• Kotelkin, A., Prikhod'ko, E. A., Cohen, J. I., Collins, P. L., Bukreyev, A. (2003). Respiratory Syncytial Virus Infection Sensitizes Cells to Apoptosis Mediated by Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand. J. Virol. 77: 9156-9172 [Abstract] [Full Text]  
• Yoshida, A., Tanaka, R., Murakami, T., Takahashi, Y., Koyanagi, Y., Nakamura, M., Ito, M., Yamamoto, N., Tanaka, Y. (2003). Induction of Protective Immune Responses against R5 Human Immunodeficiency Virus Type 1 (HIV-1) Infection in hu-PBL-SCID Mice by Intrasplenic Immunization with HIV-1-Pulsed Dendritic Cells: Possible Involvement of a Novel Factor of Human CD4+ T-Cell Origin. J. Virol. 77: 8719-8728 [Abstract] [Full Text]  
• Feng, H., Zeng, Y., Graner, M. W., Likhacheva, A., Katsanis, E. (2003). Exogenous stress proteins enhance the immunogenicity of apoptotic tumor cells and stimulate antitumor immunity. Blood 101: 245-252 [Abstract] [Full Text]  
• Feng, H., Zeng, Y., Graner, M. W., Katsanis, E. (2002). Stressed apoptotic tumor cells stimulate dendritic cells and induce specific cytotoxic T cells. Blood 100: 4108-4115 [Abstract] [Full Text]  
• Spetz, A.-L., Sorensen, A. S., Walther-Jallow, L., Wahren, B., Andersson, J., Holmgren, L., Hinkula, J. (2002). Induction of HIV-1-Specific Immunity After Vaccination with Apoptotic HIV-1/Murine Leukemia Virus-Infected Cells. J. Immunol. 169: 5771-5779 [Abstract] [Full Text]