This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Furuta, R. A.
Right arrow Articles by Fujisawa, J.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furuta, R. A.
Right arrow Articles by Fujisawa, J.-i.

 Previous Article  |  Next Article 

Journal of Virology, March 2002, p. 2703-2713, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.2703-2713.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mouse Model for the Equilibration Interaction between the Host Immune System and Human T-Cell Leukemia Virus Type 1 Gene Expression

Rika A. Furuta,1,2 Kikuya Sugiura,2,3 Shigenari Kawakita,4 Takefumi Inada,1,5 Susumu Ikehara,2,3 Tadashi Matsuda,4 and Jun-ichi Fujisawa1,2*

Department of Microbiology,1 First Department of Pathology,3 Department of Urology,4 Department of Anesthesiology,5 Transplantation Center, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan2

Received 11 July 2001/ Accepted 17 December 2001

To study the involvement of immune responses against Tax of human T-cell leukemia virus type 1 (HTLV-1) in the growth of and gene suppression in Tax-expressing tumor cells in vivo, we established a model system involving C57BL/6J mice and a syngeneic lymphoma cell line, EL4. When mice were immunized by DNA-based immunization with Tax expression plasmids, solid tumor formation upon subcutaneous inoculation of EL4 cells expressing green fluorescent protein-fused Tax (Gax) under the control of the HTLV-1 enhancer was strongly inhibited, and in vitro analysis showed that DNA immunization elicited cytotoxic T-lymphocyte (CTL) responses but not production of antibodies to Tax protein. Since EL4/Gax cells inoculated into DNA-immunized mice were not completely eradicated but were maintained as small solid tumors for a long period, there appeared to be a certain equilibrium between CTL activity and the growth of Gax-expressing cells. With such a balance, expression of the Gax gene in EL4/Gax cells was strongly suppressed. These results suggested that gene expression under the control of the HTLV-1 long terminal repeat and Tax is silenced in vivo, resulting in an equilibrium between viral expression and the host immune system. Such a balance would represent a status of persistent infection by HTLV-1 in virus-infected individuals during the latency period.

* Corresponding author. Mailing address: Department of Microbiology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8506, Japan. Phone: 81-6-6993-9430. Fax: 81-6-6993-1668. E-mail: fujisawa{at}takii.kmu.ac.jp.

Journal of Virology, March 2002, p. 2703-2713, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.2703-2713.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kinpara, S., Hasegawa, A., Utsunomiya, A., Nishitsuji, H., Furukawa, H., Masuda, T., Kannagi, M. (2009). Stromal Cell-Mediated Suppression of Human T-Cell Leukemia Virus Type 1 Expression In Vitro and In Vivo by Type I Interferon. J. Virol. 83: 5101-5108 [Abstract] [Full Text]  
  • Dewan, M. Z., Takamatsu, N., Hidaka, T., Hatakeyama, K., Nakahata, S., Fujisawa, J.-i., Katano, H., Yamamoto, N., Morishita, K. (2008). Critical Role for TSLC1 Expression in the Growth and Organ Infiltration of Adult T-Cell Leukemia Cells In Vivo. J. Virol. 82: 11958-11963 [Abstract] [Full Text]  
  • Miyazato, P., Yasunaga, J.-i., Taniguchi, Y., Koyanagi, Y., Mitsuya, H., Matsuoka, M. (2006). De Novo Human T-Cell Leukemia Virus Type 1 Infection of Human Lymphocytes in NOD-SCID, Common {gamma}-Chain Knockout Mice. J. Virol. 80: 10683-10691 [Abstract] [Full Text]  
  • Nomura, M., Ohashi, T., Nishikawa, K., Nishitsuji, H., Kurihara, K., Hasegawa, A., Furuta, R. A., Fujisawa, J.-i., Tanaka, Y., Hanabuchi, S., Harashima, N., Masuda, T., Kannagi, M. (2004). Repression of Tax Expression Is Associated both with Resistance of Human T-Cell Leukemia Virus Type 1-Infected T Cells to Killing by Tax-Specific Cytotoxic T Lymphocytes and with Impaired Tumorigenicity in a Rat Model. J. Virol. 78: 3827-3836 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»