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Journal of Virology, March 2002, p. 2579-2584, Vol. 76, No. 5
0022-538X/02/$04.00+0     DOI: 10.1128/jvi.76.5.2579-2584.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Enhancement of Hepatitis B Virus Replication by Its X Protein in Transgenic Mice

*** Zhenming Xu,1 T. S. Benedict Yen,2,3 Lanying Wu,4 Charles R. Madden,5 Wenjie Tan,1 Betty L. Slagle,5 and Jing-hsiung Ou1*

Department of Molecular Microbiology and Immunology,1 Department of Molecular Biology and Biochemistry, University of Southern California, Los Angeles, California 90033,4 Department of Pathology, University of California,2 Pathology Service, Veterans Affairs Medical Center, San Francisco, California 94121,3 Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 770305

Received 2 October 2001/ Accepted 30 November 2001

Hepatitis B virus (HBV) X gene encodes a multifunctional protein that can regulate cellular signaling pathways, interact with cellular transcription factors, and induce hepatocellular oncogenesis. In spite of its diverse activities, the precise role of the X protein in the viral life cycle of HBV remains unclear. To investigate this question, we have produced transgenic mice that carry either the wild-type HBV genome or a mutated HBV genome incapable of expressing the 16.5-kDa X protein. Our results indicate that while the X protein is not absolutely essential for HBV replication or its maturation in transgenic mice, it can enhance viral replication, apparently by activating viral gene expression. These results demonstrate a transactivation role of the X protein in HBV replication in transgenic mice.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, 2011 Zonal Ave., HMR-401, Los Angeles, CA 90033. Phone: (323) 442-1720. Fax: (323) 442-1721. E-mail: jamesou{at}hsc.usc.edu.

Journal of Virology, March 2002, p. 2579-2584, Vol. 76, No. 5
0022-538X/02/$04.00+0     DOI: 10.1128/jvi.76.5.2579-2584.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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