This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bantel-Schaal, U. Articles by Kartenbeck, J. Search for Related Content PubMed PubMed Citation Articles by Bantel-Schaal, U. Articles by Kartenbeck, J.

Endocytosis of Adeno-Associated Virus Type 5 Leads to Accumulation of Virus Particles in the Golgi Compartment

Forschungsschwerpunkt Angewandte Tumorvirologie F0400, Abteilung Pathogenitätsmechanismen,¹ Forschungsschwerpunkt Krebsentstehung und Differenzierung A0100, Abteilung für Zellbiologie, Deutsches Krebsforschungszentrum Heidelberg, 69120 Heidelberg, Germany²

Received 7 November 2001/ Accepted 19 November 2001

Among the adeno-associated virus (AAV) serotypes which are discussed as vectors for gene therapy AAV type 5 (AAV5) represents a candidate with unique advantages. To further our knowledge on AAV5-specific characteristics, we studied the entry pathway of wild-type virus in HeLa cells in the absence of helper virus by immunofluorescence and electron microscopy and by Western blot analysis. We found virus binding at the apical cell surface, especially at microvilli and, with increasing incubation time, virus accumulation at cell-cell boundaries. The different binding kinetics suggest different binding properties at apical versus lateral plasma membranes. Endocytosis of viruses was predominantly by clathrin-coated vesicles from both membrane domains; however, particles were also detected in noncoated pits. AAV5 particles were mainly routed to the Golgi area, where they could be detected within cisternae of the trans-Golgi network and within vesicles associated with cisternae and with the dictyosomal stacks of the Golgi apparatus. These data suggest that AAV5 makes use of endocytic routes that have hitherto not been described as pathways for virus entry.

This article has been cited by other articles:

• Sonntag, F., Bleker, S., Leuchs, B., Fischer, R., Kleinschmidt, J. A. (2006). Adeno-Associated Virus Type 2 Capsids with Externalized VP1/VP2 Trafficking Domains Are Generated prior to Passage through the Cytoplasm and Are Maintained until Uncoating Occurs in the Nucleus. J. Virol. 80: 11040-11054 [Abstract] [Full Text]  
• Querbes, W., O'Hara, B. A., Williams, G., Atwood, W. J. (2006). Invasion of Host Cells by JC Virus Identifies a Novel Role for Caveolae in Endosomal Sorting of Noncaveolar Ligands. J. Virol. 80: 9402-9413 [Abstract] [Full Text]  
• Mani, B., Baltzer, C., Valle, N., Almendral, J. M., Kempf, C., Ros, C. (2006). Low pH-Dependent Endosomal Processing of the Incoming Parvovirus Minute Virus of Mice Virion Leads to Externalization of the VP1 N-Terminal Sequence (N-VP1), N-VP2 Cleavage, and Uncoating of the Full-Length Genome. J. Virol. 80: 1015-1024 [Abstract] [Full Text]  
• Kronenberg, S., Bottcher, B., von der Lieth, C. W., Bleker, S., Kleinschmidt, J. A. (2005). A Conformational Change in the Adeno-Associated Virus Type 2 Capsid Leads to the Exposure of Hidden VP1 N Termini. J. Virol. 79: 5296-5303 [Abstract] [Full Text]  
• Sandalon, Z., Bruckheimer, E. M., Lustig, K. H., Rogers, L. C., Peluso, R. W., Burstein, H. (2004). Secretion of a TNFR:Fc Fusion Protein following Pulmonary Administration of Pseudotyped Adeno-Associated Virus Vectors. J. Virol. 78: 12355-12365 [Abstract] [Full Text]  
• Vihinen-Ranta, M., Suikkanen, S., Parrish, C. R. (2004). Pathways of Cell Infection by Parvoviruses and Adeno-Associated Viruses. J. Virol. 78: 6709-6714 [Full Text]  
• Hueffer, K., Palermo, L. M., Parrish, C. R. (2004). Parvovirus Infection of Cells by Using Variants of the Feline Transferrin Receptor Altering Clathrin-Mediated Endocytosis, Membrane Domain Localization, and Capsid-Binding Domains. J. Virol. 78: 5601-5611 [Abstract] [Full Text]  
• Rabinowitz, J. E., Bowles, D. E., Faust, S. M., Ledford, J. G., Cunningham, S. E., Samulski, R. J. (2004). Cross-Dressing the Virion: the Transcapsidation of Adeno-Associated Virus Serotypes Functionally Defines Subgroups. J. Virol. 78: 4421-4432 [Abstract] [Full Text]  
• Smith, A. E., Helenius, A. (2004). How Viruses Enter Animal Cells. Science 304: 237-242 [Abstract] [Full Text]  
• Imelli, N., Meier, O., Boucke, K., Hemmi, S., Greber, U. F. (2004). Cholesterol Is Required for Endocytosis and Endosomal Escape of Adenovirus Type 2. J. Virol. 78: 3089-3098 [Abstract] [Full Text]  
• Xiao, W., Warrington, K. H. Jr., Hearing, P., Hughes, J., Muzyczka, N. (2002). Adenovirus-Facilitated Nuclear Translocation of Adeno-Associated Virus Type 2. J. Virol. 76: 11505-11517 [Abstract] [Full Text]  
• Pajusola, K., Gruchala, M., Joch, H., Luscher, T. F., Yla-Herttuala, S., Bueler, H. (2002). Cell-Type-Specific Characteristics Modulate the Transduction Efficiency of Adeno-Associated Virus Type 2 and Restrain Infection of Endothelial Cells. J. Virol. 76: 11530-11540 [Abstract] [Full Text]  
• Johannes, L. (2002). The Epithelial Cell Cytoskeleton and Intracellular Trafficking: I. Shiga toxin B-subunit system: retrograde transport, intracellular vectorization, and more. Am. J. Physiol. Gastrointest. Liver Physiol. 283: G1-G7 [Abstract] [Full Text]