This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dorsch, S. Articles by Modrow, S. Search for Related Content PubMed PubMed Citation Articles by Dorsch, S. Articles by Modrow, S.

 Previous Article  |  Next Article 

Journal of Virology, February 2002, p. 2014-2018, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.2014-2018.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The VP1 Unique Region of Parvovirus B19 and Its Constituent Phospholipase A2-Like Activity

Simone Dorsch,¹ Gerhard Liebisch,² Bärbel Kaufmann,^1,3, Philipp von Landenberg,⁴ Jörg H. Hoffmann,^1, Wolfgang Drobnik,² and Susanne Modrow^1*

Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg,¹ Institut für Klinische Chemie ,² Klinik und Poliklinik für Innere Medizin I, Universitätsklinikum Resensburg, 93053 Regensburg ,⁴ Institut für Physikalische Biochemie, Universität Potsdam, 14476 Golm, Germany³

Received 11 July 2001/ Accepted 13 November 2001

Parvovirus B19 is the causative agent of erythema infectiosum. In addition, parvovirus B19 infection may be associated with other disease manifestations, namely, thrombocytopenia or granulocytopenia, spontaneous abortion or hydrops fetalis in pregnant women, acute and chronic arthritis, and systemic lupus erythematosus. Based on sequence homology data, a phospholipase A2 motif has been identified in the VP1 unique region of parvovirus B19. (Y. Li et al., J. Gen. Virol. 82:2821-2825, 2001; Z. Zadori et al., Dev. Cell 1:291-302, 2001). We have established a new in vitro assay based on electrospray ionization tandem mass spectroscopy to show that phospholipase A2 activity is present in the VP1 unique region produced in Escherichia coli and in virus-like particles consisting of combinations of VP1 and VP2 proteins expressed by recombinant baculovirus. The enzyme activity of the VP1 unique region showed typical Ca²⁺ dependency and could be inhibited by manoalide and 4-bromophenacylbromide, which bind covalently to lysine and histidine residues, respectively, as part of the active center of the enzyme. By using subfragments, we demonstrated an association between the phospholipase A2-like activity and the carboxy-terminal domain of the VP1 unique region.

Present address: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392.

Present address: Department of Biology, University of Michigan, Ann Arbor, MI 48109.

This article has been cited by other articles:

• Grieger, J. C., Johnson, J. S., Gurda-Whitaker, B., Agbandje-McKenna, M., Samulski, R. J. (2007). Surface-Exposed Adeno-Associated Virus Vp1-NLS Capsid Fusion Protein Rescues Infectivity of Noninfectious Wild-Type Vp2/Vp3 and Vp3-Only Capsids but Not That of Fivefold Pore Mutant Virions. J. Virol. 81: 7833-7843 [Abstract] [Full Text]  
• Ekman, A., Hokynar, K., Kakkola, L., Kantola, K., Hedman, L., Bonden, H., Gessner, M., Aberham, C., Norja, P., Miettinen, S., Hedman, K., Soderlund-Venermo, M. (2007). Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3. J. Virol. 81: 6927-6935 [Abstract] [Full Text]  
• Ros, C., Gerber, M., Kempf, C. (2006). Conformational Changes in the VP1-Unique Region of Native Human Parvovirus B19 Lead to Exposure of Internal Sequences That Play a Role in Virus Neutralization and Infectivity. J. Virol. 80: 12017-12024 [Abstract] [Full Text]  
• Lupescu, A., Bock, C.-T., Lang, P. A., Aberle, S., Kaiser, H., Kandolf, R., Lang, F. (2006). Phospholipase A2 Activity-Dependent Stimulation of Ca2+ Entry by Human Parvovirus B19 Capsid Protein VP1. J. Virol. 80: 11370-11380 [Abstract] [Full Text]  
• Toan, N. L., Duechting, A., Kremsner, P. G., Song, L. H., Ebinger, M., Aberle, S., Binh, V. Q., Duy, D. N., Torresi, J., Kandolf, R., Bock, C.-T. (2006). Phylogenetic analysis of human parvovirus B19, indicating two subgroups of genotype 1 in Vietnamese patients.. J. Gen. Virol. 87: 2941-2949 [Abstract] [Full Text]  
• Zhi, N., Mills, I. P., Lu, J., Wong, S., Filippone, C., Brown, K. E. (2006). Molecular and Functional Analyses of a Human Parvovirus B19 Infectious Clone Demonstrates Essential Roles for NS1, VP1, and the 11-Kilodalton Protein in Virus Replication and Infectivity.. J. Virol. 80: 5941-5950 [Abstract] [Full Text]  
• Zimmermann, P., Ritzmann, M., Selbitz, H.-J., Heinritzi, K., Truyen, U. (2006). VP1 sequences of German porcine parvovirus isolates define two genetic lineages. J. Gen. Virol. 87: 295-301 [Abstract] [Full Text]  
• Mani, B., Baltzer, C., Valle, N., Almendral, J. M., Kempf, C., Ros, C. (2006). Low pH-Dependent Endosomal Processing of the Incoming Parvovirus Minute Virus of Mice Virion Leads to Externalization of the VP1 N-Terminal Sequence (N-VP1), N-VP2 Cleavage, and Uncoating of the Full-Length Genome. J. Virol. 80: 1015-1024 [Abstract] [Full Text]  
• Farr, G. A., Cotmore, S. F., Tattersall, P. (2006). VP2 Cleavage and the Leucine Ring at the Base of the Fivefold Cylinder Control pH-Dependent Externalization of both the VP1 N Terminus and the Genome of Minute Virus of Mice. J. Virol. 80: 161-171 [Abstract] [Full Text]  
• Farr, G. A., Zhang, L.-g., Tattersall, P. (2005). Parvoviral virions deploy a capsid-tethered lipolytic enzyme to breach the endosomal membrane during cell entry. Proc. Natl. Acad. Sci. USA 102: 17148-17153 [Abstract] [Full Text]  
• Kronenberg, S., Bottcher, B., von der Lieth, C. W., Bleker, S., Kleinschmidt, J. A. (2005). A Conformational Change in the Adeno-Associated Virus Type 2 Capsid Leads to the Exposure of Hidden VP1 N Termini. J. Virol. 79: 5296-5303 [Abstract] [Full Text]  
• Tschope, C., Bock, C.-T., Kasner, M., Noutsias, M., Westermann, D., Schwimmbeck, P.-L., Pauschinger, M., Poller, W.-C., Kuhl, U., Kandolf, R., Schultheiss, H.-P. (2005). High Prevalence of Cardiac Parvovirus B19 Infection in Patients With Isolated Left Ventricular Diastolic Dysfunction. Circulation 111: 879-886 [Abstract] [Full Text]  
• Bleker, S., Sonntag, F., Kleinschmidt, J. A. (2005). Mutational Analysis of Narrow Pores at the Fivefold Symmetry Axes of Adeno-Associated Virus Type 2 Capsids Reveals a Dual Role in Genome Packaging and Activation of Phospholipase A2 Activity. J. Virol. 79: 2528-2540 [Abstract] [Full Text]  
• Vihinen-Ranta, M., Suikkanen, S., Parrish, C. R. (2004). Pathways of Cell Infection by Parvoviruses and Adeno-Associated Viruses. J. Virol. 78: 6709-6714 [Full Text]  
• Ros, C., Burckhardt, C. J., Kempf, C. (2002). Cytoplasmic Trafficking of Minute Virus of Mice: Low-pH Requirement, Routing to Late Endosomes, and Proteasome Interaction. J. Virol. 76: 12634-12645 [Abstract] [Full Text]  
• Guinness, M. E., Kenney, J. L., Reiss, M., Lacy, J. (2000). Bcl-2 Antisense Oligodeoxynucleotide Therapy of Epstein-Barr Virus-associated Lymphoproliferative Disease in Severe Combined Immunodeficient Mice. Cancer Res. 60: 5354-5358 [Abstract] [Full Text]  
• Kenney, J. L., Guinness, M. E., Curiel, T., Lacy, J. (1998). Antisense to the Epstein-Barr Virus (EBV)-Encoded Latent Membrane Protein 1 (LMP-1) Suppresses LMP-1 and Bcl-2 Expression and Promotes Apoptosis in EBV-Immortalized B Cells. Blood 92: 1721-1727 [Abstract] [Full Text]