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Journal of Virology, February 2002, p. 1980-1985, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.1980-1985.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulated Gene Expression in the Chicken Embryo by Using Replication-Competent Retroviral Vectors

Noboru Sato,1* Kenji Matsuda,1 Chie Sakuma,1 Douglas N. Foster,2 Ronald W. Oppenheim,3 and Hiroyuki Yaginuma1

Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan,1 Department of Animal Science, University of Minnesota, St. Paul, Minnesota 55108,2 Department of Neurobiology & Anatomy and Neuroscience Program, Wake Forest University School of Medicine, Winston-Salem, North Carolina 271573

Received 7 September 2001/ Accepted 19 November 2001

Rous sarcoma virus (RSV)-derived retroviral vector could efficiently deliver the green fluorescent protein (GFP), which is driven by the internal cytomegalovirus enhancer/promoter, into restricted cell populations in the chicken embryo. RSV-derived vectors coupled with the tet regulatory elements also revealed doxycycline-dependent inducible GFP expression in the chicken embryo in ovo.

* Corresponding author. Mailing address: Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. Phone: 81-24-548-2111. Fax: 81-24-549-8811. E-mail: nsato{at}fmu.ac.jp.

Journal of Virology, February 2002, p. 1980-1985, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.1980-1985.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.