This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moise, A. R.
Right arrow Articles by Jefferies, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moise, A. R.
Right arrow Articles by Jefferies, W. A.

 Previous Article  |  Next Article 

Journal of Virology, February 2002, p. 1578-1587, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.1578-1587.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Adenovirus E3-6.7K Maintains Calcium Homeostasis and Prevents Apoptosis and Arachidonic Acid Release

Alexander R. Moise,1,2 Jason R. Grant,1,2 Timothy Z. Vitalis,3 and Wilfred A. Jefferies1,2*

Biotechnology Laboratory,,1 Biomedical Research Centre, and Departments of Medical Genetics, Microbiology and Immunology, and Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3,,2 Pulmonary Research Laboratory, University of British Columbia, St. Paul's Hospital, Vancouver, British Columbia V6Z 1Y6, Canada3

Received 5 June 2001/ Accepted 9 November 2001

E3-6.7K is a small and hydrophobic membrane glycoprotein encoded by the E3 region of subgroup C adenovirus. Recently, E3-6.7K has been shown to be required for the downregulation of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptors by the adenovirus E3/10.4K and E3/14.5K complex of proteins. We demonstrate here that E3-6.7K has additional protective roles, independent of other virus proteins. In transfected Jurkat T-cell lymphoma cells, E3-6.7K was found to maintain endoplasmic reticulum-Ca2+ homeostasis and inhibit the induction of apoptosis by thapsigargin. The presence of E3-6.7K also lead to a reduction in the TNF-induced release of arachidonic acid from transfected U937 human histiocytic lymphoma cells. In addition, E3-6.7K protected cells against apoptosis induced through Fas, TNF receptor, and TRAIL receptors. Therefore, E3-6.7K confers a wide range of protective effects against both Ca2+ flux-induced and death receptor-mediated apoptosis.

* Corresponding author. Mailing address: The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. Phone: (604) 822-6961. Fax: (604) 822-6780. E-mail: wilf{at}unixg.ubc.ca.

Journal of Virology, February 2002, p. 1578-1587, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.1578-1587.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, J., Enomoto, S., Lancto, C. A., Abrahamsen, M. S., Rutherford, M. S. (2008). Inhibition of Apoptosis in Cryptosporidium parvum-Infected Intestinal Epithelial Cells Is Dependent on Survivin. Infect. Immun. 76: 3784-3792 [Abstract] [Full Text]  
  • Laxalt, A. M., Raho, N., Have, A. t., Lamattina, L. (2007). Nitric Oxide Is Critical for Inducing Phosphatidic Acid Accumulation in Xylanase-elicited Tomato Cells. J. Biol. Chem. 282: 21160-21168 [Abstract] [Full Text]  
  • Grant, J. R., Moise, A. R., Jefferies, W. A. (2007). Identification of a Novel Immunosubversion Mechanism Mediated by a Virologue of the B-Lymphocyte Receptor TACI. CVI 14: 907-917 [Abstract] [Full Text]  
  • Hilgendorf, A., Lindberg, J., Ruzsics, Z., Honing, S., Elsing, A., Lofqvist, M., Engelmann, H., Burgert, H.-G. (2003). Two Distinct Transport Motifs in the Adenovirus E3/10.4-14.5 Proteins Act in Concert to Down-modulate Apoptosis Receptors and the Epidermal Growth Factor Receptor. J. Biol. Chem. 278: 51872-51884 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»