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Journal of Virology, February 2002, p. 959-967, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.959-967.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dissociation of Genome Dimerization from Packaging Functions and Virion Maturation of Human Immunodeficiency Virus Type 1

Jun-ichi Sakuragi,1* Aikichi Iwamoto,2 and Tatsuo Shioda1

Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Suita-City, Osaka 565-0871,1 Division of Infectious Diseases, The Advanced Clinical Research Center, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan2

Received 9 July 2001/ Accepted 19 October 2001

The dimer initiation site/dimer linkage sequence (DIS/DLS) region of the human immunodeficiency virus type 1 (HIV-1) RNA genome is thought to play important roles at various stages of the virus life cycle. Recently we showed that the DIS/DLS region affects RNA-RNA interaction in intact virus particles, by demonstrating that duplication of the region in viral RNA caused the production of virus particles containing partially monomeric RNAs. We have extended this finding and succeeded for the first time in creating mutant particles which contain only monomeric RNAs without modifying any viral proteins. In terms of RNA encapsidation ability, virion density, and protein processing, the mutant particles were comparable to wild-type particles. The level of production of viral DNA by the mutant virus construct in infected cells was also comparable to that of the constructs that produced exclusively dimeric RNA, indicating that monomeric viral RNA could be the template for strand transfer. These results indicated that the RNA dimerization of HIV-1 could be separated from viral RNA packaging and was not absolutely required for RNA packaging, virion maturation, and reverse transcription.

* Corresponding author. Mailing address: Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita-City, Osaka 565-0871, Japan. Phone: 81-6-6879-8348. Fax: 81-6-6879-8347. E-mail: sakuragi{at}biken.osaka-u.ac.jp.

Journal of Virology, February 2002, p. 959-967, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.959-967.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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