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Journal of Virology, February 2002, p. 1505-1509, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.1505-1509.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Adaptation of Wild-Type Measles Virus to Tissue Culture

Diane Waku Kouomou and T. Fabian Wild*

INSERM U 404, CERVI, 69365 Lyon Cedex 07, France

Received 31 July 2001/ Accepted 24 October 2001

Measles has a host range restricted to humans and monkeys in captivity. Fresh measles virus (MV) isolates replicate readily in several human and simian B-cell lines but need a period of adaptation to other types of cells. The identification of CD46 and CD150 (SLAM) as cellular receptors for MV has helped to clarify certain aspects of the immunobiology of MV infections. We have examined the properties of an MV wild-type strain grown in the epithelial cell line Vero. After adaptation, this virus expressed high levels of both the viral glycoproteins (hemagglutinin and fusion protein) but did not induce fusion (syncytia). No changes in the amino acid sequence were found in either of the viral glycoproteins. Using several approaches, the Vero-adapted virus could not be shown to interact with CD46 either in the initiation or during the course of infection. The presence of human SLAM expressed in the Vero cells rapidly gave rise to fusion and lower yields of infectious virus.

* Corresponding author. Mailing address: INSERM U 404, CERVI - 21, Avenue Tony Garnier, 69365 Lyon Cedex 07, France. Phone: 33 4 37 28 23 92. Fax: 33 4 37 28 23 91. E-mail: wild{at}cervi-lyon.inserm.fr.

Journal of Virology, February 2002, p. 1505-1509, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.1505-1509.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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