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Journal of Virology, February 2002, p. 1379-1390, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.1379-1390.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Chaperone Hsp90 Is Important for Vaccinia Virus Growth in Cells

Jan-Jong Hung, Che-Sheng Chung, and Wen Chang*

Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China

Received 10 October 2001/ Accepted 5 November 2001

Molecular chaperones assist protein folding, and some chaperones are induced by heat, nutrient depletion, or pathogen invasion. This study investigates the role played by Hsp90 in the life cycle of vaccinia virus. The titer of vaccinia intracellular mature virions (IMV) was reduced by 2 orders of magnitude in RK13 cells treated with geldanamycin (GA), which blocks the ATPase activity of Hsp90. GA does not affect expression from the viral early promoter, but treatment with GA delays DNA replication and intermediate gene transcription and reduces expression from the viral late promoter. Vaccinia virus infection does not induce Hsp90 expression; however, intracellular distribution of Hsp90 is altered in virus-infected cells. Hsp90 is restricted to the cytoplasm of mock-infected cells; in contrast, Hsp90 is transiently associated with virosomes in virus-infected cells although it is not incorporated into IMV. In addition, Hsp90 interacts with viral core protein 4a, the mature form of the A10L gene product, in virus-infected cells. In conclusion, these results suggest that a cellular chaperone protein, Hsp90, is important for vaccinia virus growth in cultured cells and that viral core protein 4a associates with Hsp90-containing complexes in the infected cells.

* Corresponding author. Mailing address: 128, Sec. 2, Academia Rd., Nankang, Taipei 115, Taiwan, Republic of China. Phone: 886-2-2789-9230. Fax: 886-2-2782-6085. Email: mbwen{at}ccvax.sinica.edu.tw.

Journal of Virology, February 2002, p. 1379-1390, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.1379-1390.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.