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Journal of Virology, December 2002, p. 13116-13122, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13116-13122.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Terminal RNA Replication Elements in Human Parechovirus 1

Abdolrahman S. Nateri,{dagger} Pamela J. Hughes, and Glyn Stanway*

Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom

Received 15 April 2002/ Accepted 3 September 2002

To define structural elements critical for RNA replication in human parechovirus 1 (HPeV1), a replicon with chloramphenicol acetyltransferase as a reporter gene and an infectious virus cDNA clone have been used. It was observed that there are cis-acting signals required for HPeV1 replication located within the 5'-terminal 112 nucleotides of the genome and that these include two terminal stem-loops, SL-A and SL-B, together with a pseudoknot element. Significant disruption of any of these structures impaired both RNA replication and virus growth. In view of the similarity in terminal structures to several picornaviruses, such as cardioviruses and hepatoviruses, the insights generated in this work are of wider significance for understanding picornavirus replication.

* Corresponding author. Mailing address: Department of Biological Sciences, Central Campus, University of Essex, Colchester CO4 3SQ, United Kingdom. Phone: 44 1206 873308. Fax: 44 1206 872592. E-mail: stanwg{at}essex.ac.uk.

{dagger} Present address: Mammalian Genetics Laboratory, Cancer Research UK, London WC2A 3PX, United Kingdom.

Journal of Virology, December 2002, p. 13116-13122, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13116-13122.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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