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Journal of Virology, December 2002, p. 13111-13115, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13111-13115.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Retrovirus-Specific Packaging of Aminoacyl-tRNA Synthetases with Cognate Primer tRNAs

Shan Cen,1 Hassan Javanbakht,1,2 Sunghoon Kim,3 Kiyotaka Shiba,4 Rebecca Craven,5 Alan Rein,6 Karla Ewalt,7 Paul Schimmel,7 Karin Musier-Forsyth,8 and Lawrence Kleiman1,2,9*

Lady Davis Institute for Medical Research and McGill AIDS Center, Jewish General Hospital,1 Departments of Medicine,2 Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3T 1E2,9 National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea,3 Department of Cell Biology, Cancer Institute, Japanese Foundation for Cancer Research, Kami-Ikebukuro, Toshima-ku, Tokyo 170, Japan,4 Department of Biochemistry and Molecular Biology, The Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, Pennsylvania 17033,5 HIVDRP, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702,6 Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037,7 Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 554558

Received 19 June 2002/ Accepted 8 September 2002

The tRNAs used to prime reverse transcription in human immunodeficiency virus type 1 (HIV-1), Rous sarcoma virus (RSV), and Moloney murine leukemia virus (Mo-MuLV) are , tRNATrp, and tRNAPro, respectively. Using antibodies to the three cognate human aminoacyl-tRNA synthetases, we found that only lysyl-tRNA synthetase (LysRS) is present in HIV-1, only tryptophanyl-tRNA synthetase (TrpRS) is present in RSV, and neither these two synthetases nor prolyl-tRNA synthetase (ProRS) is present in Mo-MuLV. LysRS and TrpRS are present in HIV-1 and RSV at approximately 25 and 12 molecules/virion, respectively. These results support the hypothesis that, in HIV-1 and RSV, the cognate aminoacyl-tRNA synthetase may be used as the signal for targeting the selective packaging of primer tRNAs into retroviruses. The absence of ProRS in Mo-MuLV is consistent with reports that selective packaging of tRNAPro in this virus is less important for achieving optimum annealing of the primer to Mo-MuLV genomic RNA.

* Corresponding author. Mailing address: Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Cote Ste-Catherine Rd., Montreal, Quebec, Canada H3T 1E2. Phone: (514) 340-8260. Fax: (514) 340-7502. E-mail: lawrence.kleiman{at}mcgill.ca.

Journal of Virology, December 2002, p. 13111-13115, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13111-13115.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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