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Journal of Virology, December 2002, p. 13097-13100, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13097-13100.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of Neutralizing Epitopes within Structural Domain III of the West Nile Virus Envelope Protein

David W. C. Beasley and Alan D. T. Barrett*

WHO Collaborating Center for Tropical Diseases, Sealy Center for Vaccine Development, and Department of Pathology, The University of Texas Medical Branch, Galveston, Texas 77555-0609

Received 18 June 2002/ Accepted 16 September 2002

Using a panel of neutralizing monoclonal antibodies, we have mapped epitopes in domain III of the envelope protein of the New York strain of West Nile virus. The ability of monoclonal antibodies that recognize these epitopes to neutralize virus appeared to differ between lineage I and II West Nile virus strains, and epitopes were located on the upper surface of domain III at residues E307, E330, and E332.

* Corresponding author. Mailing address: Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609. Phone: (409) 772-6662. Fax: (409) 772-2500. E-mail: abarrett{at}utmb.edu.

Journal of Virology, December 2002, p. 13097-13100, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13097-13100.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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