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Journal of Virology, December 2002, p. 12908-12916, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12908-12916.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel N Gene-Associated, Temperature-Independent Resistance to the Movement of Tobacco Mosaic Virus Vectors Neutralized by a Cucumber Mosaic Virus RNA1 Transgene

Tomas Canto and Peter Palukaitis*

Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom

Received 21 May 2002/ Accepted 5 September 2002

The N gene conditions for resistance to Tobacco mosaic virus (TMV) but only below 28°C. However, a TMV-based vector expressing green fluorescent protein (TMV-GFP) showed only limited movement at 33°C in tobacco plants harboring the N gene and other genes cointrogressed from Nicotiana glutinosa. TMV-GFP moved efficiently in tobacco plants that either lacked these genes or that contained the N gene but were transgenic for RNA1 of Cucumber mosaic virus. These findings identified novel temperature-independent resistance to the movement of TMV-GFP which could be neutralized by a different viral transgene. Using the N gene and nahG gene-transgenic tobacco, we show that this novel resistance is manifested specifically by the N gene itself and operates via a pathway independent of salicylic acid.

* Corresponding author. Mailing address: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom. Phone: 44 1382 568523. Fax 44 1382 568575. E-mail: ppaluk{at}scri.sari.ac.uk.

Journal of Virology, December 2002, p. 12908-12916, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12908-12916.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez-Jara, P., Fraile, A., Canto, T., Garcia-Arenal, F. (2009). The Multiplicity of Infection of a Plant Virus Varies during Colonization of Its Eukaryotic Host. J. Virol. 83: 7487-7494 [Abstract] [Full Text]  
  • Chung, B.-N., Canto, T., Palukaitis, P. (2007). Stability of recombinant plant viruses containing genes of unrelated plant viruses. J. Gen. Virol. 88: 1347-1355 [Abstract] [Full Text]  
  • Canto, T., MacFarlane, S. A., Palukaitis, P. (2004). ORF6 of Tobacco mosaic virus is a determinant of viral pathogenicity in Nicotiana benthamiana. J. Gen. Virol. 85: 3123-3133 [Abstract] [Full Text]  


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