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Journal of Virology, December 2002, p. 12414-12422, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12414-12422.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Frequencies of Ex Vivo-Activated Human Immunodeficiency Virus Type 1-Specific Gamma-Interferon-Producing CD8+ T Cells in Infected Children Correlate Positively with Plasma Viral Load

Florence Buseyne,1* Daniel Scott-Algara,2 Françoise Porrot,1 Béatrice Corre,1 Nassima Bellal,1 Marianne Burgard,3 Christine Rouzioux,3 Stéphane Blanche,4 and Yves Rivière1

Laboratoire d'Immunopathologie Virale, URA CNRS 1930,1 Unité d'Immunohématologie-Immunopathologie, Institut Pasteur,2 Laboratoire de Virologie,3 Fédération de Pédiatrie, CHU Necker-Enfants Malades, 75015 Paris, France4

Received 24 May 2002/ Accepted 26 August 2002

HIV-specific CD8+ T cells are critical in controlling human immunodeficiency virus (HIV) replication. We present the evaluation of a gamma-interferon (IFN-{gamma})-based enzyme linked immunospot (ELISPOT) assay for the quantification of HIV-specific CD8+ T cells from HIV-infected children. We studied 20 HLA-A*0201-positive HIV-infected children. The IFN-{gamma} production in response to stimulation with two HLA-A*0201-restricted immunodominant CD8 epitopes (SLYNTVATL [SL9] in Gag and ILKEPVHGV [IV9] in Pol) was tested using the ELISPOT assay. The results were compared to labeling with the corresponding tetramers. Among the 20 children, 18 had detectable responses against the SL9 and/or the IV9 epitope using the ELISPOT assay (medians, 351 and 134 spot-forming cells/106 peripheral blood mononuclear cells, respectively). Comparison of results from the tetramer and ELISPOT assays suggests that only a fraction of HIV-specific CD8+ T cells were able to produce IFN-{gamma}. Most importantly, we found that the frequencies of IFN-{gamma}-producing CD8+ T cells were positively correlated with the viral load whereas the frequencies of tetramer-binding CD8+ T cells were not. The high sensitivity of the ELISPOT assay and the fact that this functional assay provided information different from that of tetramer labeling support its use for measurement of HIV-specific CD8+ T cells. In conclusion, our results show that the ex vivo-activated IFN-{gamma}-producing HIV-specific CD8+-T-cell subset is dependent upon continuous antigenic stimulation.

* Corresponding author. Mailing address: Laboratoire d'Immunopathologie Virale, Département de Médecine Moléculaire, BÂtiment Lwoff, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France. Phone: 33 1 45 68 88 99. Fax: 33 1 40 61 32 98. E-mail: florence{at}pasteur.fr.

Journal of Virology, December 2002, p. 12414-12422, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12414-12422.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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