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Journal of Virology, December 2002, p. 12355-12359, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12355-12359.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mucosal Immunization with Live Recombinant Bovine Respiratory Syncytial Virus (BRSV) and Recombinant BRSV Lacking the Envelope Glycoprotein G Protects against Challenge with Wild-Type BRSV

Ulrike Schmidt,1 Jörg Beyer,2 Ulf Polster,2 Laurel J. Gershwin,3 and Ursula J. Buchholz1*

Institutes of Molecular Biology,1 Infectology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, D-17498 Insel Riems, Germany,2 Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California 956163

Received 29 April 2002/ Accepted 22 August 2002

Recombinant bovine respiratory syncytial virus (rBRSV) and an rBRSV deletion mutant lacking the G gene (rBRSV{Delta}G) were characterized in calves with respect to replication competence, attenuation, and protective efficacy as live-attenuated BRSV vaccines. Both recombinant viruses were safe and induced protection against a BRSV challenge infection. rBRSV replicated efficiently in the upper respiratory tract. Intranasal immunization with rBRSV{Delta}G led to infection but not to mucosal virus replication. Neutralizing antibodies were induced by rBRSV and rBRSV{Delta}G. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV.

* Corresponding author. Mailing address: Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany. Phone: 49 38351 7215. Fax: 49 38351 7275. E-mail: buchholz{at}rie.bfav.de.

Journal of Virology, December 2002, p. 12355-12359, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12355-12359.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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