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Journal of Virology, December 2002, p. 12349-12354, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12349-12354.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Targeted Derepression of the Human Immunodeficiency Virus Type 1 Long Terminal Repeat by Pyrrole-Imidazole Polyamides

Jason J. Coull,1 Guocheng He,1 Christian Melander,2 Victor C. Rucker,2 Peter B. Dervan,2 and David M. Margolis1,3,4*

Division of Infectious Diseases, Department of Medicine,1 Department of Cell and Molecular Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390,3 Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125,2 North Texas Veterans Health Care Systems, Dallas, Texas 752164

Received 4 June 2002/ Accepted 28 August 2002

The host factor LSF represses the human immunodeficiency virus type 1 long terminal repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-imidazole polyamides targeted to the LTR can specifically block LSF binding both in vitro and within cells via direct access to chromatin, resulting in increased LTR expression.

* Corresponding author. Mailing address: University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Y9-206a, Dallas, TX 75390-9113. Phone: (214) 648-3593. Fax: (214) 648-0231. E-mail: david.margolis{at}UTSouthwestern.edu.

Journal of Virology, December 2002, p. 12349-12354, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12349-12354.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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