This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luhr, K. M.
Right arrow Articles by Kristensson, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luhr, K. M.
Right arrow Articles by Kristensson, K.

 Previous Article  |  Next Article 

Journal of Virology, December 2002, p. 12259-12264, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12259-12264.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Processing and Degradation of Exogenous Prion Protein by CD11c+ Myeloid Dendritic Cells In Vitro

Katarina M. Luhr,1 Robert P. A. Wallin,2 Hans-Gustaf Ljunggren,2 Peter Löw,1 Albert Taraboulos,3 and Krister Kristensson1*

Department of Neuroscience,1 Microbiology and Tumor Biology Center, Karolinska Institutet, SE-171 77 Stockholm, Sweden,2 Department of Molecular Biology, Hebrew University Hadassah Medical School, 91120 Jerusalem, Israel3

Received 28 March 2002/ Accepted 19 August 2002

The immune system plays an important role in facilitating the spread of prion infections from the periphery to the central nervous system. CD11c+ myeloid dendritic cells (DC) could, due to their subepithelial location and their migratory capacity, be early targets for prion infection and contribute to the spread of infection. In order to analyze mechanisms by which these cells may affect prion propagation, we studied in vitro the effect of exposing such DC to scrapie-infected GT1-1 cells, which produce the scrapie prion protein PrPSc. In this system, the DC efficiently engulfed the infected GT1-1 cells. Unexpectedly, PrPSc, which is generally resistant to protease digestion, was processed and rapidly degraded. Based on this observation we speculate that CD11c+ DC may play a dual role in prion infections: on one hand they may facilitate neuroinvasion by transfer of the infectious agent as suggested from in vivo studies, but on the other hand they may protect against the infection by causing an efficient degradation of PrPSc. Thus, the migrating and highly proteolytic CD11c+ myeloid DC may affect the balance between propagation and clearance of PrPSc in the organism.

* Corresponding author. Mailing address: Department of Neuroscience, Karolinska Institutet, Retzius väg 8, Stockholm SE-171 77, Sweden. Phone: 46-8-728 78 25. Fax: 46-8-32 53 25. E-mail: Krister.Kristensson{at}neuro.ki.se.

Journal of Virology, December 2002, p. 12259-12264, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12259-12264.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Flores-Langarica, A., Sebti, Y., Mitchell, D. A., Sim, R. B., MacPherson, G. G. (2009). Scrapie Pathogenesis: The Role of Complement C1q in Scrapie Agent Uptake by Conventional Dendritic Cells. J. Immunol. 182: 1305-1313 [Abstract] [Full Text]  
  • Spinner, D. S., Cho, I. S., Park, S. Y., Kim, J. I., Meeker, H. C., Ye, X., LaFauci, G., Kerr, D. J., Flory, M. J., Kim, B. S., Kascsak, R. B., Wisniewski, T., Levis, W. R., Schuller-Levis, G. B., Carp, R. I., Park, E., Kascsak, R. J. (2008). Accelerated Prion Disease Pathogenesis in Toll-Like Receptor 4 Signaling-Mutant Mice. J. Virol. 82: 10701-10708 [Abstract] [Full Text]  
  • Wacnik, P. W., Luhr, K. M., Hill, R. H., Ljunggren, H.-G., Kristensson, K., Svensson, M. (2008). Cannabinoids Affect Dendritic Cell (DC) Potassium Channel Function and Modulate DC T Cell Stimulatory Capacity. J. Immunol. 181: 3057-3066 [Abstract] [Full Text]  
  • Raymond, C. R., Aucouturier, P., Mabbott, N. A. (2007). In Vivo Depletion of CD11c+ Cells Impairs Scrapie Agent Neuroinvasion from the Intestine. J. Immunol. 179: 7758-7766 [Abstract] [Full Text]  
  • Levavasseur, E., Metharom, P., Dorban, G., Nakano, H., Kakiuchi, T., Carnaud, C., Sarradin, P., Aucouturier, P. (2007). Experimental scrapie in 'plt' mice: an assessment of the role of dendritic-cell migration in the pathogenesis of prion diseases. J. Gen. Virol. 88: 2353-2360 [Abstract] [Full Text]  
  • Martinez del Hoyo, G., Lopez-Bravo, M., Metharom, P., Ardavin, C., Aucouturier, P. (2006). Prion Protein Expression by Mouse Dendritic Cells Is Restricted to the Nonplasmacytoid Subsets and Correlates with the Maturation State. J. Immunol. 177: 6137-6142 [Abstract] [Full Text]  
  • Rybner-Barnier, C., Jacquemot, C., Cuche, C., Dore, G., Majlessi, L., Gabellec, M.-M., Moris, A., Schwartz, O., Di Santo, J., Cumano, A., Leclerc, C., Lazarini, F. (2006). Processing of the bovine spongiform encephalopathy-specific prion protein by dendritic cells.. J. Virol. 80: 4656-4663 [Abstract] [Full Text]  
  • Murali, J., Jayakumar, R. (2006). Lymphocyte toxicity of prion fragments.. J Biochem 139: 329-338 [Abstract] [Full Text]  
  • Jacquemot, C., Cuche, C., Dormont, D., Lazarini, F. (2005). High Incidence of Scrapie Induced by Repeated Injections of Subinfectious Prion Doses. J. Virol. 79: 8904-8908 [Abstract] [Full Text]  
  • Horonchik, L., Tzaban, S., Ben-Zaken, O., Yedidia, Y., Rouvinski, A., Papy-Garcia, D., Barritault, D., Vlodavsky, I., Taraboulos, A. (2005). Heparan Sulfate Is a Cellular Receptor for Purified Infectious Prions. J. Biol. Chem. 280: 17062-17067 [Abstract] [Full Text]  
  • Khalili-Shirazi, A., Quaratino, S., Londei, M., Summers, L., Tayebi, M., Clarke, A. R., Hawke, S. H., Jackson, G. S., Collinge, J. (2005). Protein Conformation Significantly Influences Immune Responses to Prion Protein. J. Immunol. 174: 3256-3263 [Abstract] [Full Text]  
  • Mohan, J., Bruce, M. E., Mabbott, N. A. (2005). Neuroinvasion by Scrapie following Inoculation via the Skin Is Independent of Migratory Langerhans Cells. J. Virol. 79: 1888-1897 [Abstract] [Full Text]  
  • Luhr, K. M., Nordstrom, E. K., Low, P., Ljunggren, H.-G., Taraboulos, A., Kristensson, K. (2004). Scrapie Protein Degradation by Cysteine Proteases in CD11c+ Dendritic Cells and GT1-1 Neuronal Cells. J. Virol. 78: 4776-4782 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»