This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, Y.-W.
Right arrow Articles by Chen, S.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, Y.-W.
Right arrow Articles by Chen, S.-H.

 Previous Article  |  Next Article 

Journal of Virology, December 2002, p. 12242-12249, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12242-12249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Virus Replication and Cytokine Production in Dengue Virus-Infected Human B Lymphocytes

Yu-Wen Lin,1 Kuan-Ju Wang,1 Huan-Yao Lei,1 Yee-Shin Lin,1 Trai-Ming Yeh,2 Hsiao-Sheng Liu,1 Ching-Chuan Liu,3 and Shun-Hua Chen1*

Department of Microbiology and Immunology,1 Department of Medical Technology,2 Department of Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China3

Received 7 May 2002/ Accepted 23 August 2002

Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that DV actively replicated in B cells. Virus infection also induced B cells to produce interleukin-6 and tumor necrosis factor alpha, which have been previously implicated in virus pathogenesis. In addition, a heterologous antibody was able to enhance both virus and cytokine production in B cells. Furthermore, the levels of virus replication, antibody-enhanced virus replication, and cytokine responses observed in B cells were not statistically different from those in monocytes. These results suggest that B cells may play an important role in DV pathogenesis.


* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China. Phone: 886-6-2353535, ext. 5633. Fax: 886-6-208-2705. E-mail: shunhua{at}mail.ncku.edu.tw.


Journal of Virology, December 2002, p. 12242-12249, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12242-12249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Lin, Y.-W., Wang, S.-W., Tung, Y.-Y., Chen, S.-H. (2009). Enterovirus 71 Infection of Human Dendritic Cells. Exp. Biol. Med. 234: 1166-1173 [Abstract] [Full Text]  
  • Gregory, S. M., West, J. A., Dillon, P. J., Hilscher, C., Dittmer, D. P., Damania, B. (2009). Toll-like receptor signaling controls reactivation of KSHV from latency. Proc. Natl. Acad. Sci. USA 106: 11725-11730 [Abstract] [Full Text]  
  • Warke, R. V., Becerra, A., Zawadzka, A., Schmidt, D. J., Martin, K. J., Giaya, K., Dinsmore, J. H., Woda, M., Hendricks, G., Levine, T., Rothman, A. L., Bosch, I. (2008). Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells. J. Gen. Virol. 89: 1605-1615 [Abstract] [Full Text]  
  • Warke, R. V., Martin, K. J., Giaya, K., Shaw, S. K., Rothman, A. L., Bosch, I. (2008). TRAIL Is a Novel Antiviral Protein against Dengue Virus. J. Virol. 82: 555-564 [Abstract] [Full Text]  
  • Blackley, S., Kou, Z., Chen, H., Quinn, M., Rose, R. C., Schlesinger, J. J., Coppage, M., Jin, X. (2007). Primary Human Splenic Macrophages, but Not T or B Cells, Are the Principal Target Cells for Dengue Virus Infection In Vitro. J. Virol. 81: 13325-13334 [Abstract] [Full Text]  
  • Wati, S., Li, P., Burrell, C. J., Carr, J. M. (2007). Dengue Virus (DV) Replication in Monocyte-Derived Macrophages Is Not Affected by Tumor Necrosis Factor Alpha (TNF-{alpha}), and DV Infection Induces Altered Responsiveness to TNF-{alpha} Stimulation. J. Virol. 81: 10161-10171 [Abstract] [Full Text]  
  • Sariol, C. A., Munoz-Jordan, J. L., Abel, K., Rosado, L. C., Pantoja, P., Giavedoni, L., Rodriguez, I. V., White, L. J., Martinez, M., Arana, T., Kraiselburd, E. N. (2007). Transcriptional Activation of Interferon-Stimulated Genes but Not of Cytokine Genes after Primary Infection of Rhesus Macaques with Dengue Virus Type 1. CVI 14: 756-766 [Abstract] [Full Text]  
  • Goncalvez, A. P., Engle, R. E., St. Claire, M., Purcell, R. H., Lai, C.-J. (2007). Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention. Proc. Natl. Acad. Sci. USA 104: 9422-9427 [Abstract] [Full Text]  
  • Takhampunya, R., Padmanabhan, R., Ubol, S. (2006). Antiviral action of nitric oxide on dengue virus type 2 replication.. J. Gen. Virol. 87: 3003-3011 [Abstract] [Full Text]  
  • McClintock, J. Y., Wagner, E. M. (2005). Role of IL-6 in systemic angiogenesis of the lung. J. Appl. Physiol. 99: 861-866 [Abstract] [Full Text]  
  • Lin, C.-F., Chiu, S.-C., Hsiao, Y.-L., Wan, S.-W., Lei, H.-Y., Shiau, A.-L., Liu, H.-S., Yeh, T.-M., Chen, S.-H., Liu, C.-C., Lin, Y.-S. (2005). Expression of Cytokine, Chemokine, and Adhesion Molecules during Endothelial Cell Activation Induced by Antibodies against Dengue Virus Nonstructural Protein 1. J. Immunol. 174: 395-403 [Abstract] [Full Text]  
  • Hung, J.-J., Hsieh, M.-T., Young, M.-J., Kao, C.-L., King, C.-C., Chang, W. (2004). An External Loop Region of Domain III of Dengue Virus Type 2 Envelope Protein Is Involved in Serotype-Specific Binding to Mosquito but Not Mammalian Cells. J. Virol. 78: 378-388 [Abstract] [Full Text]