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Journal of Virology, December 2002, p. 12242-12249, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12242-12249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Virus Replication and Cytokine Production in Dengue Virus-Infected Human B Lymphocytes

Yu-Wen Lin,1 Kuan-Ju Wang,1 Huan-Yao Lei,1 Yee-Shin Lin,1 Trai-Ming Yeh,2 Hsiao-Sheng Liu,1 Ching-Chuan Liu,3 and Shun-Hua Chen1*

Department of Microbiology and Immunology,1 Department of Medical Technology,2 Department of Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China3

Received 7 May 2002/ Accepted 23 August 2002

Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that DV actively replicated in B cells. Virus infection also induced B cells to produce interleukin-6 and tumor necrosis factor alpha, which have been previously implicated in virus pathogenesis. In addition, a heterologous antibody was able to enhance both virus and cytokine production in B cells. Furthermore, the levels of virus replication, antibody-enhanced virus replication, and cytokine responses observed in B cells were not statistically different from those in monocytes. These results suggest that B cells may play an important role in DV pathogenesis.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China. Phone: 886-6-2353535, ext. 5633. Fax: 886-6-208-2705. E-mail: shunhua{at}mail.ncku.edu.tw.

Journal of Virology, December 2002, p. 12242-12249, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12242-12249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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