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Journal of Virology, November 2002, p. 11770-11774, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11770-11774.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Altered DNA Mutation Spectrum in Aflatoxin B1-Treated Transgenic Mice That Express the Hepatitis B Virus X Protein

Charles R. Madden,1 Milton J. Finegold,2 and Betty L. Slagle1*

Department of Molecular Virology and Microbiology,1 Department of Pathology, Baylor College of Medicine, Houston, Texas 770302

Received 30 April 2002/ Accepted 14 August 2002

Humans chronically infected with hepatitis B virus (HBV) are at further risk of liver cancer upon exposure to dietary aflatoxin B1 (AFB1), a carcinogenic product of the mold Aspergillus flavus. For the present study, we utilized double-transgenic mice (ATX mice) that express the HBV X protein (HBx) and possess a bacteriophage lambda transgene to evaluate the in vivo effect of HBx expression on AFB1-induced DNA mutations. The expression of HBx correlated with a 24% increase in mutation frequency overall and an approximately twofold increase in the incidence of G/C-to-T/A transversion mutations following AFB1 exposure. These results are consistent with a model in which expression of HBx during chronic HBV infection may contribute to the development of hepatocellular carcinoma following exposure to environmental carcinogens.

* Corresponding author. Mailing address: Department of Molecular Virology and Microbiology, Mailstop BCM-385, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030-3411. Phone: (713) 798-3006. Fax: (713) 798-5075. E-mail: bslagle{at}bcm.tmc.edu.

Journal of Virology, November 2002, p. 11770-11774, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11770-11774.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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