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Journal of Virology, November 2002, p. 11744-11747, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11744-11747.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Reverse Genetics Approach for Recovery of Recombinant Influenza B Viruses Entirely from cDNA

David Jackson,1 Andrew Cadman,2 Thomas Zurcher,2 and Wendy S. Barclay1*

University of Reading, Whiteknights, Reading RG6 6AJ,1 GlaxoSmithKline Medicine Research Centre, Stevenage SG1 2NY, United Kingdom2

Received 16 July 2002/ Accepted 12 August 2002

The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

* Corresponding author. Mailing address: School of Animal and Microbial Sciences, University of Reading, P.O. Box 228, Whiteknights, Reading RG6 6AJ, United Kingdom. Phone: 44-118-9316368. Fax: 44-118-9316671. E-mail: w.s.barclay{at}reading.ac.uk.

Journal of Virology, November 2002, p. 11744-11747, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11744-11747.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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