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Journal of Virology, November 2002, p. 11738-11743, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11738-11743.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Porcine Endogenous Retrovirus {gamma} pro-pol Nucleotide Sequences

Nikolai Klymiuk,1,2 Mathias Müller,2 Gottfried Brem,1,3 and Bernhard Aigner1,2*

ApoGene Biotechnologie, D-86567 Hilgertshausen, Germany,1 Institut für Tierzucht und Genetik, Veterinärmedizinische Universität Wien,2 Ludwig-Boltzmann-Institut für Immuno-, Zyto- und Molekulargenetische Forschung Wien, A-1210 Vienna, Austria3

Received 2 July 2002/ Accepted 19 August 2002

Endogenous retroviral sequences in the pig genome (PERV) represent a potential infectious risk in xenotransplantation. All known infectious PERV have been asssigned to the PERV {gamma}1 family, consisting of the subfamilies A, B, and C. The aim of the study was the concise examination of PERV {gamma} by the analysis of the retroviral pro-pol sequences. The analysis of 52 pro-pol clones amplified in this study revealed eight PERV {gamma} families. In addition to four already-described families ({gamma}1, {gamma}4, {gamma}5, {gamma}6), four novel families ({gamma}7, {gamma}8, {gamma}9, {gamma}10) were identified. Quantitative analysis of the novel PERV {gamma} sequences in selected breeds revealed variations in the endogenous retroviral load. Open reading frames (ORF) in the amplified proviral fragment were only found for PERV {gamma}1. In addition, novel ORF-containing PERV {gamma}1 clones consisting of hybrid sequences were revealed. Sequence comparison from published full-length PERV {gamma}1 clones of the PERV subfamilies A, B, and C resulted in a lack of strict correlation of the classification of pro-pol and env. The results indicated the occurrence of causative recombination events between retroviral genomes. Thus, our study on PERV {gamma} provides new data for the evaluation and selection of pigs intended to be used in xenotransplantation.

* Corresponding author. Mailing address: Institut für Tierzucht und Genetik, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Vienna. Phone: 43-1-25077-5604. Fax: 43-1-25077-5693. E-mail: bernhard.aigner{at}i122server.vu-wien.ac.at.

Journal of Virology, November 2002, p. 11738-11743, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11738-11743.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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