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Journal of Virology, October 2002, p. 10553-10558, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10553-10558.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification and Expression of Immunogenic Proteins of a Disease-Associated Marine Turtle Herpesvirus

Sadie S. Coberley,¹ Richard C. Condit,² Lawrence H. Herbst,³ and Paul A. Klein^4*

Interdisciplinary Program in Biomedical Sciences,¹ Department of Molecular Genetics and Microbiology,² Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida 32610,⁴ Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461³

Received 24 April 2002/ Accepted 9 July 2002

Herpesviruses are associated with several diseases of marine turtles, including lung-eye-trachea disease (LETD) and fibropapillomatosis. Two approaches were used to identify immunodominant antigens of LETV, the LETD-associated herpesvirus. The first approach targeted glycoprotein B, which is known to be immunogenic and neutralizing in other species. The second strategy identified LETV proteins recognized on Western blots by antibodies in immune green turtle plasma. A 38-kDa protein was resolved by two-dimensional gel electrophoresis, sequenced, and identified as a scaffolding protein encoded by the overlapping open reading frames of UL26 and UL26.5. Glycoprotein B and the scaffolding protein were cloned and expressed in Escherichia coli. The expressed proteins were recognized on Western blots by antibodies in immune green turtle plasma. Phylogenetic studies based on UL26, DNA polymerase, and glycoprotein B revealed that LETV clusters with the alphaherpesviruses.

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* Corresponding author. Mailing address: Department of Pathology, Immunology, and Laboratory Medicine, Box 100275, University of Florida, Gainesville, FL 32610-0275. Phone: (352) 392-2608. Fax: (352) 392-1619. E-mail: paklein{at}ufl.edu.

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Journal of Virology, October 2002, p. 10553-10558, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10553-10558.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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