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Journal of Virology, October 2002, p. 10346-10355, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10346-10355.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Exchange of the C-Terminal Part of VP3 from Very Virulent Infectious Bursal Disease Virus Results in an Attenuated Virus with a Unique Antigenic Structure

Hein J. Boot,* A. Agnes H. M. ter Huurne, Arjan J. W. Hoekman, Jan M. Pol, Arno L. J. Gielkens, and Ben P. H. Peeters

Institute for Animal Science and Health, Lelystad, The Netherlands

Received 8 April 2002/ Accepted 8 July 2002

Infectious bursal disease virus (IBDV) is the major viral pathogen in the poultry industry. Live attenuated serotype 1 vaccine strains are commonly used to protect susceptible chickens during their first 6 weeks of life. Wild-type serotype 1 IBDV strains are highly pathogenic only in chickens, whereas serotype 2 strains are apathogenic in chickens and other birds. Here we describe the replacement of the genomic double-stranded RNA (dsRNA) encoding the N- or C-terminal part of VP3 of serotype 1 very virulent IBDV (vvIBDV) (isolate D6948) with the corresponding part of serotype 2 (isolate TY89) genomic dsRNA. The modified virus containing the C-terminal part of serotype 2 VP3 significantly reduced the virulence in specific-pathogen-free chickens, without affecting the distinct bursa tropism of serotype 1 IBDV strains. Furthermore, by using serotype-specific antibodies we were able to distinguish bursas infected with wild-type vvIBDV from bursas infected with the modified vvIBDV. We are currently evaluating the potential of this recombinant strain as an attenuated live vaccine that induces a unique serological response (i.e., an IBDV marker vaccine).

* Corresponding author. Mailing address: ID-Lelystad, P. O. Box 65, NL-8200 AB Lelystad, The Netherlands. Phone: 31 320 238 695. Fax: 31 320 238 668. E-mail: H.J.Boot{at}id.wag-ur.nl.

Journal of Virology, October 2002, p. 10346-10355, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10346-10355.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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