Previous Article | Next Article 
Journal of Virology, October 2002, p. 10338-10345, Vol. 76, No. 20
0022-538X/02/$04.00+0 DOI: 10.1128/JVI.76.20.10338-10345.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Phenotypic and Functional Alterations of Dendritic Cells Induced by Human Herpesvirus 6 Infection
Miki Kakimoto, Atsuhiko Hasegawa, Shigeru Fujita, and Masaki Yasukawa*
First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan
Received 19 February 2002/
Accepted 12 July 2002
Human herpesvirus 6 (HHV-6) has a tropism for T lymphocytes and monocytes/macrophages, suggesting that HHV-6 infection affects the immunosurveillance system. In the present study, we investigated the HHV-6-induced phenotypic and functional alterations of dendritic cells (DCs), which are professional antigen-presenting cells. HHV-6 infection of monocyte-derived immature DCs appeared to induce the up-regulation of CD80, CD83, CD86, and HLA class I and class II molecules, suggesting that HHV-6 infection induces the maturation of DCs. In addition, the antigen capture capacity of DCs was found to decrease following infection with HHV-6. In contrast to up-regulation of mature-DC-associated surface molecules on HHV-6-infected DCs, their capacity for presentation of alloantigens and exogenous virus antigens to T lymphocytes decreased significantly from that of uninfected DCs. In contrast, there appeared to be no reduction in the capacity for presentation of an HLA class II-binding peptide to the peptide-specific CD4+ T lymphocytes. These data indicate that HHV-6 infection induces phenotypic alterations and impairs the antigen presentation capacity of DCs. The present data also suggest that the dysfunction of HHV-6-infected DCs is attributable mainly to impairment of the antigen capture and intracellular antigen-processing pathways.
* Corresponding author. Mailing address: First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan. Phone: 81-89-960-5296. Fax: 81-89-960-5299. E-mail: yasukawa{at}m.ehime-u.ac.jp.
Journal of Virology, October 2002, p. 10338-10345, Vol. 76, No. 20
0022-538X/02/$04.00+0 DOI: 10.1128/JVI.76.20.10338-10345.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Harman, A. N., Wilkinson, J., Bye, C. R., Bosnjak, L., Stern, J. L., Nicholle, M., Lai, J., Cunningham, A. L.
(2006). HIV Induces Maturation of Monocyte-Derived Dendritic Cells and Langerhans Cells. J. Immunol.
177: 7103-7113
[Abstract]
[Full Text]
-
Flano, E., Kayhan, B., Woodland, D. L., Blackman, M. A.
(2005). Infection of Dendritic Cells by a {gamma}2-Herpesvirus Induces Functional Modulation. J. Immunol.
175: 3225-3234
[Abstract]
[Full Text]
-
Smith, A. P., Paolucci, C., Di Lullo, G., Burastero, S. E., Santoro, F., Lusso, P.
(2005). Viral Replication-Independent Blockade of Dendritic Cell Maturation and Interleukin-12 Production by Human Herpesvirus 6. J. Virol.
79: 2807-2813
[Abstract]
[Full Text]
-
Opsahl, M. L., Kennedy, P. G. E.
(2005). Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter. Brain
128: 516-527
[Abstract]
[Full Text]
-
De Bolle, L., Naesens, L., De Clercq, E.
(2005). Update on Human Herpesvirus 6 Biology, Clinical Features, and Therapy. Clin. Microbiol. Rev.
18: 217-245
[Abstract]
[Full Text]
-
Fantuzzi, L., Purificato, C., Donato, K., Belardelli, F., Gessani, S.
(2004). Human Immunodeficiency Virus Type 1 gp120 Induces Abnormal Maturation and Functional Alterations of Dendritic Cells: a Novel Mechanism for AIDS Pathogenesis. J. Virol.
78: 9763-9772
[Abstract]
[Full Text]
-
Niiya, H., Azuma, T., Jin, L., Uchida, N., Inoue, A., Hasegawa, H., Fujita, S., Tohyama, M., Hashimoto, K., Yasukawa, M.
(2004). Transcriptional downregulation of DC-SIGN in human herpesvirus 6-infected dendritic cells. J. Gen. Virol.
85: 2639-2642
[Abstract]
[Full Text]
-
Borenstein, R., Singer, O., Moseri, A., Frenkel, N.
(2004). Use of Amplicon-6 Vectors Derived from Human Herpesvirus 6 for Efficient Expression of Membrane-Associated and -Secreted Proteins in T Cells. J. Virol.
78: 4730-4743
[Abstract]
[Full Text]
-
Morrow, G., Slobedman, B., Cunningham, A. L., Abendroth, A.
(2003). Varicella-Zoster Virus Productively Infects Mature Dendritic Cells and Alters Their Immune Function. J. Virol.
77: 4950-4959
[Abstract]
[Full Text]
Copyright © 2002 by the American Society for Microbiology. All rights reserved.