This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loeb, D. D.
Right arrow Articles by Tian, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loeb, D. D.
Right arrow Articles by Tian, R.

 Previous Article  |  Next Article 

Journal of Virology, October 2002, p. 10195-10202, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10195-10202.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Secondary Structure That Contains the 5' and 3' Splice Sites Suppresses Splicing of Duck Hepatitis B Virus Pregenomic RNA

Daniel D. Loeb,* Amanda A. Mack, and Ru Tian

McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706

Received 10 May 2002/ Accepted 12 July 2002

Pregenomic RNA (pgRNA) plays two major roles in the hepadnavirus life cycle. It is the mRNA for two proteins required for DNA replication, C and P, and it is the template for reverse transcription. pgRNA is a terminally redundant transcript whose synthesis does not involve RNA splicing. For duck hepatitis B virus (DHBV), a spliced RNA is derived from pgRNA by removal of a single intron. The mechanism for the simultaneous cytoplasmic accumulation of unspliced (pgRNA) and spliced RNA was not known. We found that mutations within two regions of the DHBV genome reduced the level of pgRNA while increasing the level of spliced RNA. One region is near the 5' end of pgRNA (region A), while the second is near the middle of pgRNA (region B). Inspection of the DHBV nucleotide sequence indicated that region A could base pair with region B. The 5' and 3' splice sites of the intron of the spliced RNA are within regions A and B, respectively. Substitutions that disrupted the predicted base pairing reduced the accumulation of pgRNA and increased the accumulation of spliced RNA. Restoration of base pairing, albeit mutant in sequence, resulted in restoration of pgRNA accumulation with a decrease in the level of spliced RNA. Our data are consistent with a model in which splicing of the pgRNA is suppressed by a secondary structure between regions A and B that occludes the splicing machinery from modifying pgRNA.

* Corresponding author. Mailing address: McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, 1400 University Ave., Madison, WI 53706. Phone: (608) 262-1260. Fax: (608) 262-2824. E-mail: loeb{at}oncology.wisc.edu.

Journal of Virology, October 2002, p. 10195-10202, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10195-10202.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jablonski, J. A., Buratti, E., Stuani, C., Caputi, M. (2008). The Secondary Structure of the Human Immunodeficiency Virus Type 1 Transcript Modulates Viral Splicing and Infectivity. J. Virol. 82: 8038-8050 [Abstract] [Full Text]  
  • Schwalbe, M., Ohlenschlager, O., Marchanka, A., Ramachandran, R., Hafner, S., Heise, T., Gorlach, M. (2008). Solution structure of stem-loop {alpha} of the hepatitis B virus post-transcriptional regulatory element. Nucleic Acids Res 36: 1681-1689 [Abstract] [Full Text]  
  • Grigoras, I., Timchenko, T., Gronenborn, B. (2008). Transcripts encoding the nanovirus master replication initiator proteins are terminally redundant. J. Gen. Virol. 89: 583-593 [Abstract] [Full Text]  
  • Heise, T., Sommer, G., Reumann, K., Meyer, I., Will, H., Schaal, H. (2006). The hepatitis B virus PRE contains a splicing regulatory element. Nucleic Acids Res 34: 353-363 [Abstract] [Full Text]  
  • Buratti, E., Baralle, F. E. (2004). Influence of RNA Secondary Structure on the Pre-mRNA Splicing Process. Mol. Cell. Biol. 24: 10505-10514 [Full Text]  
  • Froissart, R., Uzest, M., Ruiz-Ferrer, V., Drucker, M., Hebrard, E., Hohn, T., Blanc, S. (2004). Splicing of Cauliflower mosaic virus 35S RNA serves to downregulate a toxic gene product. J. Gen. Virol. 85: 2719-2726 [Abstract] [Full Text]  
  • Ostrow, K. M., Loeb, D. D. (2004). Underrepresentation of the 3' Region of the Capsid Pregenomic RNA of Duck Hepatitis B Virus. J. Virol. 78: 2179-2186 [Abstract] [Full Text]  
  • Buratti, E., Muro, A. F., Giombi, M., Gherbassi, D., Iaconcig, A., Baralle, F. E. (2004). RNA Folding Affects the Recruitment of SR Proteins by Mouse and Human Polypurinic Enhancer Elements in the Fibronectin EDA Exon. Mol. Cell. Biol. 24: 1387-1400 [Abstract] [Full Text]  
  • Habig, J. W., Loeb, D. D. (2003). Template Switches during Plus-Strand DNA Synthesis of Duck Hepatitis B Virus Are Influenced by the Base Composition of the Minus-Strand Terminal Redundancy. J. Virol. 77: 12412-12420 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»