This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strauss, E. G.
Right arrow Articles by Strauss, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strauss, E. G.
Right arrow Articles by Strauss, J. H.

 Previous Article  |  Next Article 

Journal of Virology, October 2002, p. 10188-10194, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10188-10194.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Genetic Evidence that the Hydrophobic Anchors of Glycoproteins E2 and E1 Interact during Assembly of Alphaviruses

Ellen G. Strauss,* Edith M. Lenches, and James H. Strauss

Division of Biology, California Institute of Technology, Pasadena, California 91125

Received 23 April 2002/ Accepted 8 July 2002

Chimeric alphaviruses in which the 6K and glycoprotein E1 moieties of Sindbis virus are replaced with those of Ross River virus grow very poorly, but upon passage, adapted variants arise that grow >100 times better. We have sequenced the entire domain encoding the E2, 6K, and E1 proteins of a number of these adapted variants and found that most acquired two amino acid changes, which had cumulative effects. In three independent passage series, amino acid 380 of E2, which is in the transmembrane domain, was mutated from the original isoleucine to serine in two instances and to valine once. We have now changed this residue to seven others by site-directed mutagenesis and tested the effects of these mutations on the growth of both the chimera [SIN(RRE1)] and of parental Sindbis. These results indicate that the transmembrane domains of glycoproteins E2 and E1 of alphaviruses interact in a sequence-dependent manner and that this interaction is required for efficient budding and assembly of infectious virions.

* Corresponding author. Mailing address: Division of Biology, 156-29, California Institute of Technology, Pasadena, CA 91125. Phone: (626) 395-4903. Fax: (626) 449-0756. E-mail: strausse{at}cco.caltech.edu.

Journal of Virology, October 2002, p. 10188-10194, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10188-10194.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Whitehurst, C. B., Soderblom, E. J., West, M. L., Hernandez, R., Goshe, M. B., Brown, D. T. (2007). Location and Role of Free Cysteinyl Residues in the Sindbis Virus E1 and E2 Glycoproteins. J. Virol. 81: 6231-6240 [Abstract] [Full Text]  
  • Pfeffer, M., Foster, J. E., Edwards, E. A., Brown, M. B., Komar, N., Brown, C. R. (2006). Phylogenetic Analysis of Buggy Creek Virus: Evidence for Multiple Clades in the Western Great Plains, United States of America. Appl. Environ. Microbiol. 72: 6886-6893 [Abstract] [Full Text]  
  • Hernandez, R., Sinodis, C., Horton, M., Ferreira, D., Yang, C., Brown, D. T. (2003). Deletions in the Transmembrane Domain of a Sindbis Virus Glycoprotein Alter Virus Infectivity, Stability, and Host Range. J. Virol. 77: 12710-12719 [Abstract] [Full Text]  
  • Sjoberg, M., Garoff, H. (2003). Interactions between the Transmembrane Segments of the Alphavirus E1 and E2 Proteins Play a Role in Virus Budding and Fusion. J. Virol. 77: 3441-3450 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»