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Journal of Virology, October 2002, p. 9991-9999, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.9991-9999.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The African Swine Fever Virus Protein j4R Binds to the Alpha Chain of Nascent Polypeptide-Associated Complex

Lynnette C. Goatley,¹ Steve R. F. Twigg,^2, James E. Miskin,^1, Paul Monaghan,¹ René St-Arnaud,³ Geoffrey L. Smith,² and Linda K. Dixon^1*

Institute for Animal Health, Pirbright, Woking, Surrey GU24 ONF,¹ Faculty of Medicine, Wright Fleming Institute, Imperial CollegeSt. Mary's Campus, London W2 1PG 3, United Kingdom,² Genetics Unit, Shriners Hospital for Children, Montreal, H3G 1A6 Quebec, Canada³

Received 5 June 2002/ Accepted 19 June 2002

The African swine fever virus (ASFV) j4R protein is expressed late during the virus replication cycle and is present in both the nucleus and the cytoplasm of infected cells. By using the yeast two-hybrid system, direct binding, and coprecipitation from cells, we showed that the j4R protein binds to the alpha chain of nascent polypeptide-associated complex (NAC). Confocal microscopy indicated that a proportion of j4R and NAC interact in areas close to the plasma membrane, as well as through the cytoplasm in cells. In vitro binding studies suggested that binding of j4R to NAC did not interfere with the binding of - and ßNAC subunits (the BTF3 transcription factor).

Present address: Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom.

Present address: Oxford BioMedica, Oxford OX4 4GA, United Kingdom.

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