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Journal of Virology, October 2002, p. 10069-10073, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.10069-10073.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Pregenomic RNA Is Required for Efficient Incorporation of Pol Polyprotein into Foamy Virus Capsids

Martin Heinkelein,1,2 Cordula Leurs,3 Matthias Rammling,2 Katrin Peters,1,2 Helmut Hanenberg,3 and Axel Rethwilm1,2*

Institut für Virologie, Medizinische Fakultät "Carl Gustav Carus," Technische Universität Dresden, Dresden,1 Institut für Virologie und Immunbiologie, Universität Würzburg, Würzburg,2 Kinderklinik, Universität Düsseldorf, Düsseldorf, Germany3

Received 29 March 2002/ Accepted 27 June 2002

The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly.

* Corresponding author. Mailing address: Institut für Virologie, Medizinische Fakultät "Carl Gustav Carus," Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany. Phone: (49) 351-458-6200. Fax: (49) 351-458-6314. E-mail: Axel.Rethwilm{at}mailbox.tu-dresden.de.

Journal of Virology, October 2002, p. 10069-10073, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.10069-10073.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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