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Journal of Virology, October 2002, p. 10050-10055, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.10050-10055.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Reversal by Dithiothreitol Treatment of the Block in Murine Leukemia Virus Maturation Induced by Disulfide Cross-Linking

Stephen Campbell,1,{dagger} Masamichi Oshima,1,{ddagger} Jane Mirro,1 Kunio Nagashima,2 and Alan Rein1*

HIV Drug Resistance Program,1 Image Analysis Laboratory, SAIC Frederick, National Cancer Institute—Frederick, Frederick, Maryland 217022

Received 13 August 2001/ Accepted 15 June 2002

We previously reported that if murine leukemia virus particles are produced in the presence of the mild oxidizing agent disulfide-substituted benzamide-2, they fail to undergo the normal process of virus maturation. We now show that treatment of these immature particles with a reducing agent (dithiothreitol) induces their maturation in vitro, as evidenced by proteolytic cleavage of Gag, Gag-Pol, and Env proteins and by their morphology. The identification of partial cleavage products in these particles suggests the sequence with which the cleavages occur under these conditions. This may be a useful experimental system for further analysis of retroviral maturation under controlled conditions in vitro.

* Corresponding author. Mailing address: National Cancer Institute—Frederick, P.O. Box B, Frederick, MD 21702. Phone: (301) 846-1361. Fax: (301) 846-7146. E-mail: rein{at}ncifcrf.gov.

{dagger} Present address: Protein Expression Laboratory, Cornell University, Ithaca, NY 14853.

{ddagger} Present address: National Institute of Infectious Diseases, Tokyo, Japan.

Journal of Virology, October 2002, p. 10050-10055, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.10050-10055.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Feher, A., Boross, P., Sperka, T., Miklossy, G., Kadas, J., Bagossi, P., Oroszlan, S., Weber, I. T., Tozser, J. (2006). Characterization of the murine leukemia virus protease and its comparison with the human immunodeficiency virus type 1 protease.. J. Gen. Virol. 87: 1321-1330 [Abstract] [Full Text]  
  • Oshima, M., Muriaux, D., Mirro, J., Nagashima, K., Dryden, K., Yeager, M., Rein, A. (2004). Effects of Blocking Individual Maturation Cleavages in Murine Leukemia Virus Gag. J. Virol. 78: 1411-1420 [Abstract] [Full Text]  


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