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Journal of Virology, September 2002, p. 9551-9555, Vol. 76, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.18.9551-9555.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Human Cytomegalovirus Ribonucleotide Reductase Homolog UL45 Is Dispensable for Growth in Endothelial Cells, as Determined by a BAC-Cloned Clinical Isolate of Human Cytomegalovirus with Preserved Wild-Type Characteristics

Gabriele Hahn,1* Hanna Khan,1 Fausto Baldanti,2 Ulrich H. Koszinowski,1 M. Grazia Revello,2 and Giuseppe Gerna2

Max von Pettenkofer Institut für Virologie, Ludwig-Maximilians-Universität München, 80336 Munich, Germany,1 Servizio di Virologia, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, 27100 Pavia, Italy2

Received 29 March 2002/ Accepted 15 June 2002

An endothelial cell-tropic and leukotropic human cytomegalovirus (HCMV) clinical isolate was cloned as a fusion-inducing factor X-bacterial artificial chromosome in Escherichia coli, and the ribonucleotide reductase homolog UL45 was deleted. Reconstituted virus RVFIX and RV{Delta}UL45 grew equally well in human fibroblasts and human endothelial cells. Thus, UL45 is dispensable for growth of HCMV in both cell types.

* Corresponding author. Mailing address: Max von Pettenkofer Institut für Virologie, Pettenkoferstr. 9A, 80336 Munich, Germany. Phone: 49-89-5160-5270. Fax: 49-89-5160-5292. E-mail: ghahn{at}m3401.mpk.med.uni-muenchen.de.

Journal of Virology, September 2002, p. 9551-9555, Vol. 76, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.18.9551-9555.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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