Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids

doi:10.1128/JVI.76.17.8939-8952.2002

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Journal of Virology, September 2002, p. 8939-8952, Vol. 76, No. 17
0022-538X/02/$04.00+0 DOI: 10.1128/JVI.76.17.8939-8952.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ultrastructural Localization of the Herpes Simplex Virus Type 1 U_L31, U_L34, and U_S3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids

Ashley E. Reynolds,¹ Elizabeth G. Wills,¹ Richard J. Roller,² Brent J. Ryckman,² and Joel D. Baines¹^*

Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853,¹ Department of Microbiology, University of Iowa, Iowa City, Iowa 52242²

Received 1 April 2002/ Accepted 4 June 2002

The wild-type U_L31, U_L34, and U_S3 proteins localized on nuclearmembranes and perinuclear virions; the U_S3 protein was alsoon cytoplasmic membranes and extranuclear virions. The U_L31and U_L34 proteins were not detected in extracellular virions.U_S3 deletion caused (i) virion accumulation in nuclear membraneinvaginations, (ii) delayed virus production onset, and (iii)reduced peak virus titers. These data support the herpes simplexvirus type 1 deenvelopment-reenvelopment model of virion egressand suggest that the U_S3 protein plays an important, but nonessential,role in the egress pathway.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Cornell University, Veterinary Medical Center (VMC) C5 131, Ithaca, NY 14853. Phone: (607) 253-3385. Fax: (607) 253-3384. E-mail: jdb11{at}cornell.edu.

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