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Journal of Virology, August 2002, p. 8494-8503, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.8494-8503.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Recovery of Virulent and RNase-Negative Attenuated Type 2 Bovine Viral Diarrhea Viruses from Infectious cDNA Clones

Christiane Meyer,1,{dagger} Martina von Freyburg,1 Knut Elbers,2 and Gregor Meyers1*

Institute of Immunology, Federal Research Centre for Virus Diseases of Animals, D-72001 Tübingen,1 Boehringer Ingelheim Vetmedica GmbH, D-55216 Ingelheim am Rhein, Germany2

Received 23 January 2002/ Accepted 7 May 2002

Cloned cDNA derived from the genome of the virulent type 2 bovine viral diarrhea virus (BVDV) strain NY'93/C was sequenced and served for establishment of the infectious cDNA clone pKANE40A. Virus recovered from pKANE40A exhibited growth characteristics similar to those of wild-type BVDV NY'93/C and proved to be clinically indistinguishable from the wild-type virus in animal experiments. A virus mutant in which the RNase residing in the viral glycoprotein Erns was inactivated, revealed an attenuated phenotype. The plasmid pKANE40A represents the first infectious cDNA clone established for a type 2 BVDV and offers a variety of new approaches to analyze the mechanisms of BVDV-induced disease in cattle.

* Corresponding author. Mailing address: Federal Research Centre for Virus Diseases of Animals, Institute of Immunology, Paul-Ehrlich-Str. 28, D-72076 Tübingen, Germany. Phone: 49 7071-9670. Fax: 49 7071-967303. E-mail: gregor.meyers{at}tue.bfav.de.

{dagger} Present address: bioScreen European Veterinary Disease Management Center GmbH, D-48149 Münster, Germany.

Journal of Virology, August 2002, p. 8494-8503, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.8494-8503.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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