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Journal of Virology, August 2002, p. 8040-8049, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.8040-8049.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Survey of Transcript Expression in Rainbow Trout Leukocytes Reveals a Major Contribution of Interferon-Responsive Genes in the Early Response to a Rhabdovirus Infection

Caroline O'Farrell,^, Nikta Vaghefi,^, Monique Cantonnet, Bénédicte Buteau, Pierre Boudinot, and Abdenour Benmansour^*

Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France

Received 19 February 2002/ Accepted 10 May 2002

Virus infections induce changes in the expression of host cell genes. A global knowledge of these modifications should help to better understand the virus/host cell interactions. To obtain a more comprehensive view of the rainbow trout response to a viral infection, we used the subtractive suppressive hybridization methodology in the viral hemorrhagic septicemia model of infection. We infected rainbow trout leukocytes with viral hemorrhagic septicemia virus (VHSV), and total RNA from infected and mock-infected cells was compared at 40 h postinfection. Twenty-four virus-induced genes were ultimately retrieved from the subtracted cDNA library, and their differential expression was further confirmed by semiquantitative reverse transcription-PCR and Northern blot analysis. Among these sequences, three were already described as VHSV-induced genes. Eight sequences with known homologs were extended to full-length cDNA using 5' and 3' rapid amplification of cDNA ends, and they were subsequently divided into three functional subsets. Four genes were homologous to mammalian interferon responsive genes, three were similar to chemo-attractant molecules (CXC chemokine, galectin), and two had nucleic acid binding domains. All of the virus-induced genes were also induced by rainbow trout interferon, indicating that the interferon pathway is the predominant component of the anti-VHSV response. They were also expressed in vivo in experimentally infected fish, indicating their biological relevance in natural infection.

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* Corresponding author. Mailing address: Institut National de la Recherche Agronomique, Unité de Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas Cedex, France. Phone: 33 1 34 65 25 90. Fax: 33 1 34 65 25 91. E-mail: abdenour{at}jouy.inra.fr.

Present address: Molecular Biology, American Type Culture Collection, Manassas, VA 20110-2209.

Present address: INSERM U 457, Hopital Robert Debré, 75019 Paris, France.

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Journal of Virology, August 2002, p. 8040-8049, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.8040-8049.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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