This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jayakar, H. R.
Right arrow Articles by Whitt, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jayakar, H. R.
Right arrow Articles by Whitt, M. A.

 Previous Article  |  Next Article 

Journal of Virology, August 2002, p. 8011-8018, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.8011-8018.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of Two Additional Translation Products from the Matrix (M) Gene That Contribute to Vesicular Stomatitis Virus Cytopathology

Himangi R. Jayakar,{dagger} and Michael A. Whitt*

Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163

Received 13 November 2001/ Accepted 3 May 2002

The matrix (M) protein of vesicular stomatitis virus (VSV) is a multifunctional protein that is responsible for condensation of the ribonucleocapsid core during virus assembly and also plays a critical role in virus budding. The M protein is also responsible for most of the cytopathic effects (CPE) observed in infected cells. VSV CPE include inhibition of host gene expression, disablement of nucleocytoplasmic transport, and disruption of the host cytoskeleton, which results in rounding of infected cells. In this report, we show that the VSV M gene codes for two additional polypeptides, which we have named M2 and M3. These proteins are synthesized from downstream methionines in the same open reading frame as the M protein (which we refer to here as M1) and lack the first 32 (M2) or 50 (M3) amino acids of M1. Infection of cells with a recombinant virus that does not express M2 and M3 (M33,51A) resulted in a delay in cell rounding, but virus yield was not affected. Transient expression of M2 and M3 alone caused cell rounding similar to that with the full-length M1 protein, suggesting that the cell-rounding function of the M protein does not require the N-terminal 50 amino acids. To determine if M2 and M3 were sufficient for VSV-mediated CPE, both M2 and M3 were expressed from a separate cistron in a VSV mutant background that readily establishes persistent infections and that normally lacks CPE. Infection of cells with the recombinant virus that expressed M2 and M3 resulted in cell rounding indistinguishable from that with the wild-type recombinant virus. These results suggest that M2 and M3 are important for cell rounding and may play an important role in viral cytopathogenesis. To our knowledge, this is first report of the multiple coding capacities of a rhabdovirus matrix gene.

* Corresponding author. Mailing address: Department of Molecular Sciences, 858 Madison Ave., University of Tennessee Health Science Center, Memphis, TN 38163. Phone: (901) 448-4634. Fax: (901) 448-8462. E-mail: mwhitt{at}utmem.edu.

{dagger} Present address: GTx, Inc., Memphis, TN 38163.

Journal of Virology, August 2002, p. 8011-8018, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.8011-8018.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Abe, T., Kaname, Y., Wen, X., Tani, H., Moriishi, K., Uematsu, S., Takeuchi, O., Ishii, K. J., Kawai, T., Akira, S., Matsuura, Y. (2009). Baculovirus Induces Type I Interferon Production through Toll-Like Receptor-Dependent and -Independent Pathways in a Cell-Type-Specific Manner. J. Virol. 83: 7629-7640 [Abstract] [Full Text]  
  • Remold, S. K., Rambaut, A., Turner, P. E. (2008). Evolutionary Genomics of Host Adaptation in Vesicular Stomatitis Virus. Mol Biol Evol 25: 1138-1147 [Abstract] [Full Text]  
  • Zhang, X., Green, T. J., Tsao, J., Qiu, S., Luo, M. (2008). Role of Intermolecular Interactions of Vesicular Stomatitis Virus Nucleoprotein in RNA Encapsidation. J. Virol. 82: 674-682 [Abstract] [Full Text]  
  • Cooper, D., Wright, K. J., Calderon, P. C., Guo, M., Nasar, F., Johnson, J. E., Coleman, J. W., Lee, M., Kotash, C., Yurgelonis, I., Natuk, R. J., Hendry, R. M., Udem, S. A., Clarke, D. K. (2008). Attenuation of Recombinant Vesicular Stomatitis Virus-Human Immunodeficiency Virus Type 1 Vaccine Vectors by Gene Translocations and G Gene Truncation Reduces Neurovirulence and Enhances Immunogenicity in Mice. J. Virol. 82: 207-219 [Abstract] [Full Text]  
  • Irie, T., Carnero, E., Okumura, A., Garcia-Sastre, A., Harty, R. N. (2007). Modifications of the PSAP region of the matrix protein lead to attenuation of vesicular stomatitis virus in vitro and in vivo. J. Gen. Virol. 88: 2559-2567 [Abstract] [Full Text]  
  • Abe, T., Kaname, Y., Hamamoto, I., Tsuda, Y., Wen, X., Taguwa, S., Moriishi, K., Takeuchi, O., Kawai, T., Kanto, T., Hayashi, N., Akira, S., Matsuura, Y. (2007). Hepatitis C Virus Nonstructural Protein 5A Modulates the Toll-Like Receptor-MyD88-Dependent Signaling Pathway in Macrophage Cell Lines. J. Virol. 81: 8953-8966 [Abstract] [Full Text]  
  • Clarke, D. K., Nasar, F., Lee, M., Johnson, J. E., Wright, K., Calderon, P., Guo, M., Natuk, R., Cooper, D., Hendry, R. M., Udem, S. A. (2007). Synergistic Attenuation of Vesicular Stomatitis Virus by Combination of Specific G Gene Truncations and N Gene Translocations. J. Virol. 81: 2056-2064 [Abstract] [Full Text]  
  • Publicover, J., Ramsburg, E., Robek, M., Rose, J. K. (2006). Rapid Pathogenesis Induced by a Vesicular Stomatitis Virus Matrix Protein Mutant: Viral Pathogenesis Is Linked to Induction of Tumor Necrosis Factor Alpha. J. Virol. 80: 7028-7036 [Abstract] [Full Text]  
  • Plesa, G., McKenna, P. M., Schnell, M. J., Eisenlohr, L. C. (2006). Immunogenicity of cytopathic and noncytopathic viral vectors.. J. Virol. 80: 6259-6266 [Abstract] [Full Text]  
  • Reuter, T., Weissbrich, B., Schneider-Schaulies, S., Schneider-Schaulies, J. (2006). RNA Interference with Measles Virus N, P, and L mRNAs Efficiently Prevents and with Matrix Protein mRNA Enhances Viral Transcription.. J. Virol. 80: 5951-5957 [Abstract] [Full Text]  
  • Tsai, C.-W., Redinbaugh, M. G., Willie, K. J., Reed, S., Goodin, M., Hogenhout, S. A. (2005). Complete Genome Sequence and In Planta Subcellular Localization of Maize Fine Streak Virus Proteins. J. Virol. 79: 5304-5314 [Abstract] [Full Text]  
  • Garbutt, M., Liebscher, R., Wahl-Jensen, V., Jones, S., Moller, P., Wagner, R., Volchkov, V., Klenk, H.-D., Feldmann, H., Stroher, U. (2004). Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses. J. Virol. 78: 5458-5465 [Abstract] [Full Text]  
  • Vassilaki, N., Mavromara, P. (2003). Two Alternative Translation Mechanisms Are Responsible for the Expression of the HCV ARFP/F/Core+1 Coding Open Reading Frame. J. Biol. Chem. 278: 40503-40513 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»