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Journal of Virology, August 2002, p. 7996-8002, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.7996-8002.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Proteolytic Processing of a Serotype 8 Human Astrovirus ORF2 Polyprotein

Ernesto Méndez,* Teresa Fernández-Luna, Susana López, Martha Méndez-Toss, and Carlos F. Arias

Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico

Received 14 January 2002/ Accepted 7 May 2002

Astroviruses require the proteolytic cleavage of the capsid protein to infect the host cell. Here we describe the processing pathway of the primary translation product of the structural polyprotein (ORF2) encoded by a human astrovirus serotype 8 (strain Yuc8). The primary translation product of ORF2 is of approximately 90 kDa, which is subsequently cleaved to yield a 70-kDa protein (VP70) which is assembled into the viral particles. Limited trypsin treatment of purified particles containing VP70 results in the generation of polypeptides VP41 and VP28, which are then further processed to proteins of 38.5, 35, and 34 kDa and 27, 26, and 25 kDa, respectively. VP34, VP27 and VP25 are the predominant proteins in fully cleaved virions, which correlate with the highest level of infectivity. Processing of the VP41 protein to yield VP38.5 to VP34 polypeptides occurred at its carboxy terminus, as suggested by immunoblot analysis using hyperimmune sera to different regions of the ORF2, while processing of VP28 to generate VP27 and VP25 occurred at its carboxy and amino terminus, respectively, as determined by immunoblot, as well as by N-terminal sequencing of those products. Based on these data, the processing pathway for the 90-kDa primary product of astrovirus Yuc8 ORF2 is presented.

* Corresponding author. Mailing address: Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico. Phone: (52)(7)329-1612. Fax: (52)(7)317-2388. E-mail: ernesto{at}ibt.unam.mx.

Journal of Virology, August 2002, p. 7996-8002, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.7996-8002.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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